caa a22 4500 467893489 CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-2762-5 doi (NATIONALLICENCE)springer-10.1007/s11103-005-2762-5 Fine Mapping and in silico Isolation of the EUI1 Gene Controlling Upper Internode Elongation in Rice [Elektronische Daten] [Hongli Ma, Shubiao Zhang, Lan Ji, Hongbo Zhu, Shulan Yang, Xuanjun Fang, Rencui Yang] Upper internode elongation in rice is an important agronomic trait. Well-known mutants with an elongated uppermost internode (eui) are important germplasms for developing unsheathed-panicle male-sterile lines in hybrid rice breeding. We finely mapped the eui1 gene and identified its candidate gene using in silico analysis based on previous research work and rice genomic sequence data. The rice eui1 gene was mapped to two overlapping BAC clones, OSJNBa0095J22 and OSJNBb0099O15, between the markers AC40 and AC46, that were 0.64 cM apart and spanned approximately 152kb. A simple sequence repeat (SSR) marker AC41 that cosegregated with eui1 was located in an intron of a putative cytochrome P450-related gene. In silico analysis suggested that this encoded the cytochrome CYP714D1. Allelic sequencing confirmed that EUI1 corresponded to this P450 gene. A gamma ray-induced eui1 mutant carried a deletion in exon II of the EUI1 gene, and resulted in a frame-shift deletion that produced a truncated polypeptide. We conclude that the EUI1 gene controlling the upper internode elongation in rice is 9804bp long, and comprises two exons and one intron. The length of the cDNA is 1931bp containing a 1734bp ORF, a 110bp 5′-UTR and a 87bp 3′-UTR. The ORF encodes an unknown 577 amino acid functional protein, that appears to be a member of the cytochrome P450 family. Springer, 2006 elongated uppermost internode ( eui ) nationallicence EUI1 gene nationallicence fine mapping nationallicence in silico cloning nationallicence rice ( Oryza sativa L) nationallicence BAC : bacteria artificial chromosome nationallicence bp : base pair nationallicence cDNA : complementary DNA nationallicence cM : centi Morgan nationallicence dNTPs : deoxyribonucleotide triphosphates nationallicence EST : expressed sequence tags nationallicence eui : elongated uppermost internode nationallicence NCBI : national center of biotechnology information nationallicence ORF : open reading frame nationallicence PA64 eS1 : Pei'ai 64 eS1 nationallicence PCR : polymerase chain reaction nationallicence PDB : protein data bank nationallicence SSR : simple sequence repeat nationallicence STS : sequence tagged site nationallicence UTR : untranslated region nationallicence XQZ eB1 : Xieqingzao eB1 nationallicence ZH15 : Zhonghua 15 nationallicence Ma Hongli Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Zhang Shubiao Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Ji Lan Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Zhu Hongbo Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Yang Shulan Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Fang Xuanjun Hainan Institute of Tropical Agricultural Resources, 572025, Sanya, China aut Yang Rencui Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 87-94 0167-4412 60:1<87 2006 60 11103 https://doi.org/10.1007/s11103-005-2762-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-2762-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Hongli Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Zhang Shubiao Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Ji Lan Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Zhu Hongbo Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Yang Shulan Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Fang Xuanjun Hainan Institute of Tropical Agricultural Resources, 572025, Sanya, China aut NATIONALLICENCE 700 1- Yang Rencui Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/1(2006-01-01), 87-94 0167-4412 60:1<87 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer