caa a22 4500 467893543 CHVBK 20180406152755.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s11103-005-4065-2 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4065-2 Quantitative Analyses of Cell Division in Plants [Elektronische Daten] [Fabio Fiorani, Gerrit Beemster] At the molecular level regulatory interactions between cell cycle genes are being uncovered rapidly, but less progress is made in unravelling how these molecular events regulate growth processes at the level of cells and of the whole organism. The main obstacle is the absence of a set of analytical tools that are powerful enough to determine pertinent parameters and, at the same time, relatively easy to use by non-specialized laboratories. Appropriate methodology to obtain this type of data has been pioneered in the first half of the last century and is now commonly defined as ‘kinematic analysis'. Unfortunately, the laborious nature of these analyses and the relatively complex numerical methods used, have limited their use to only a handful of specialized research groups. In this article we attempt to present an accessible entry to this methodology, particularly in terms of the mathematical framework. We start describing the simplest possible system, i.e., a virtually homogenous cell suspension culture. Then, we outline the analysis of dicotyledonous leaves, root tips, monocotyledonous leaves, and finally shoot apical meristems. For each of these systems we discuss the details of the calculation of cell division parameters such as cell cycle duration, size of the meristem and number of cells contained in it, which enables answering fundamental questions about the relative contribution of differences in cell production and cell size to variation in growth. In addition, we discuss the assumptions and limitations of these and alternative methodologies with the aim to facilitate the choice of appropriate analyses depending on the specific research question. Springer, 2006 cell division nationallicence cell cycle nationallicence kinematic analysis nationallicence meristem nationallicence growth nationallicence BrdU : Br-deoxy-uridine nationallicence d : cell division rate nationallicence D avg : average division rate nationallicence DAPI : 4′-6-diamino-phenolindole nationallicence DIC : differential interference contrast nationallicence E : organ elongation rate nationallicence F : cell flux nationallicence F mat : cell flux in the mature zone nationallicence GFP : green fluorescent protein nationallicence GUS : β-glucuronidase reporter gene nationallicence l : cell length nationallicence l mat : mature cell length nationallicence N div : number of cells in the division zone nationallicence N el : number of cells in the ‘elongation-only' zone nationallicence P : cell production nationallicence r : relative cell expansion rate nationallicence T div : residence time in the division zone nationallicence T el : residence time in the ‘elongation-only' zone nationallicence V : displacement velocity nationallicence ρ : cell density nationallicence Fiorani Fabio Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB)/University of Ghent, Technologiepark 927, Ghent, Belgium aut Beemster Gerrit Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB)/University of Ghent, Technologiepark 927, Ghent, Belgium aut Plant Molecular Biology Kluwer Academic Publishers 60/6(2006-04-01), 963-979 0167-4412 60:6<963 2006 60 11103 https://doi.org/10.1007/s11103-005-4065-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4065-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fiorani Fabio Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB)/University of Ghent, Technologiepark 927, Ghent, Belgium aut NATIONALLICENCE 700 1- Beemster Gerrit Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB)/University of Ghent, Technologiepark 927, Ghent, Belgium aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/6(2006-04-01), 963-979 0167-4412 60:6<963 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer