caa a22 4500 467893640 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4208-5 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4208-5 Cloning and Characterization of a Second form of the Rice Adenine Phosphoribosyl Transferase Gene ( OsAPT2 ) and its Association with TGMS [Elektronische Daten] [Chun-Jiang Zhou, Jun Li, Ji-Cheng Zou, Feng-Shan Liang, Chun-Jiang Ye, De-Min Jin, Man-Li Weng, Bin Wang] A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-PCR analysis indicated that the OsAPT2 transcript in the young panicles of ‘Annong S-1' is down-regulated at 29°C, the critical temperature for induction of ‘Annong S-1' fertility conversion. Since the panicle is likely the thermo-sensitive organ at the early stages of pollen fertility alternation, the observed heat-induced change in the OsAPT2 expression pattern in young panicles may mediate, at least in part, thermo-sensitive genic male sterility (TGMS) in ‘Annong S-1'. An antisense strategy was used to suppress the expression of the OsAPT2 homolog in Arabidopsis, and the obtained homozygous transgenic plants contained lower AMP content, displayed lower pollen germination rates and exhibited some abnormalities in leaf phenotypes and flowering timing. These data suggest that OsAPT2 is likely to be involved in TGMS in the rice line ‘Annong S-1'. Springer, 2006 adenine phosphoribosyl transferase (APRT) nationallicence Arabidopsis thaliana nationallicence Oryzasativa nationallicence thermo-sensitive genic male sterility (TGMS) nationallicence Zhou Chun-Jiang State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Li Jun State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Zou Ji-Cheng State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Liang Feng-Shan State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Ye Chun-Jiang State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Jin De-Min State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Weng Man-Li State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Wang Bin State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 365-376 0167-4412 60:3<365 2006 60 11103 https://doi.org/10.1007/s11103-005-4208-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4208-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Chun-Jiang State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Li Jun State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Zou Ji-Cheng State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Liang Feng-Shan State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Ye Chun-Jiang State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Jin De-Min State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Weng Man-Li State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Wang Bin State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 365-376 0167-4412 60:3<365 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer