caa a22 4500 467893659 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4429-7 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4429-7 Lower Levels of Transgene Silencing in Roots is Associatedwith Reduced DNA Methylation Levels at Non-Symmetrical Sites but not at Symmetrical Sites [Elektronische Daten] [Ida Andika, Hideki Kondo, Muhammad Rahim, Tetsuo Tamada] Transgene transcripts were recently shown to accumulate at higher levels in roots, relative to leaves, of silenced-transgenic Nicotiana benthamiana plants and to be inversely related with the accumulation of small interfering RNAs (siRNAs), suggesting that RNA silencing is less active in roots than in leaves (Andika et al., 2005. Mol. Plant-Microbe Interact. 18: 194). Here we show that the lower transgene RNA silencing activity in roots was associated with lower transgene methylation levels at non-symmetrical CpNpN context but not at symmetrical CpG or CpNpG context in three sets of transformant plants with different exogenous genes. In contrast, such a difference between roots and leaves was not observed for the Tnt1 retrotransposon: no Tnt1 transcript was detected in roots or in leaves of N. benthamiana, while equal levels of Tnt1-derived siRNA accumulation and Tnt1 methylation were found. From our data and previously reported information, we suggest that roots have less of an activity that acts at the step of generation of siRNAs. Springer, 2006 DNA methylation nationallicence RNA silencing nationallicence root tissue nationallicence transposon silencing nationallicence BNYVV : Beet necrotic yellow vein virus nationallicence C : cytosine nationallicence DIG : digoxigenin nationallicence dsRNA : double-stranded RNA nationallicence GFP : green fluorescent protein nationallicence LTR : long terminal repeat nationallicence Nos : nopaline synthase nationallicence ORF : open reading frame nationallicence PTGS : post-transcriptional gene silencing nationallicence RdDM : RNA-directed DNA methylation nationallicence RdRP : RNA-dependent RNA polymerase nationallicence RNAi : RNA interfering nationallicence RTD : readthrough domain nationallicence siRNA : small interfering RNA nationallicence VIGS : virus-induced gene silencing nationallicence Andika Ida Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut Kondo Hideki Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut Rahim Muhammad Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut Tamada Tetsuo Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 423-435 0167-4412 60:3<423 2006 60 11103 https://doi.org/10.1007/s11103-005-4429-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4429-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Andika Ida Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut NATIONALLICENCE 700 1- Kondo Hideki Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut NATIONALLICENCE 700 1- Rahim Muhammad Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut NATIONALLICENCE 700 1- Tamada Tetsuo Research Institute for Bioresources, Okayama University, 710-0046, Kurashiki, Okayama, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 423-435 0167-4412 60:3<423 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer