caa a22 4500 467893667 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4813-3 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4813-3 Expression of 1-Aminocyclopropane-1-Carboxylate (ACC) Oxidase Genes During the Development of Vegetative Tissues in White Clover ( Trifolium repens L.) is Regulated by Ontological Cues [Elektronische Daten] [Balance Chen, Michael McManus*] Four 5′ flanking sequences, comprising the 5′-UTR and upstream promoter region, have been isolated and cloned from the 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family of white clover (Trifolium repens L.), and designated TR-ACO1p, TR-ACO2p, TR-ACO3p and TR-ACO4p. Southern analysis confirmed that these sequences correspond to four distinct genes. The four corresponding genomic sequences have also been isolated and each shown to be comprised of four exons interspersed by three introns. The expression pattern, in vivo, directed by all four 5′ flanking sequences during leaf development has been examined using GUS fusions and transformation into white clover. Here, the TR-ACO1 5′ flanking sequence directs highest expression in the apical tissues, axillary buds, and leaf petiolules in younger tissues and then declines in the ageing tissues, while the TR-ACO2 5′ flanking sequence directs expression in both younger, mature green and in ontologically ageing tissue. The TR-ACO3 and TR-ACO4 5′ flanking sequences direct more expression in the ontological older tissues, including the axillary buds and leaf petiolules. The TR-ACO1 5′ flanking sequence directed expression in the ground meristem and newly emerged leaf tissue at the apical bud of the stolon, but all four 5′ flanking sequences directed expression in the ground meristem tissue of axillary buds, vascular tissue, pith and cortex of the internode and node, and the cortex and vascular tissue of the leaf petiolule, with the primacy of each promoter determined by the ontological age of the tissues. These data suggest that in vegetative tissue development of white clover, the primary cues for the transcriptional regulation of the ACO gene family are ontological in nature. Springer, 2006 ACC oxidase nationallicence ethylene nationallicence regulatory sequences nationallicence Trifolium repens (L.) nationallicence vegetative development nationallicence white clover nationallicence ACO : 1-aminocyclopropane-1-carboxylate oxidase nationallicence Chen Balance Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand aut McManus* Michael Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 451-467 0167-4412 60:3<451 2006 60 11103 https://doi.org/10.1007/s11103-005-4813-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4813-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Balance Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- McManus* Michael Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 451-467 0167-4412 60:3<451 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer