caa a22 4500 467893675 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4110-1 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4110-1 The Homeobox Gene GLABRA2 Affects Seed Oil Content in Arabidopsis [Elektronische Daten] [Bo Shen, Kerstin Sinkevicius, David Selinger, Mitchell Tarczynski] Despite a good understanding of genes involved in oil biosynthesis in seed, the mechanism(s) that controls oil accumulation is still not known. To identify genes that control oil accumulation in seed, we have developed a simple screening method to isolate Arabidopsis seed oil mutants. The method includes an initial screen for seed density followed by a seed oil screen using an automated Nuclear Magnetic Resonance (NMR). Using this method, we isolated ten low oil mutants and one high oil mutant. The high oil mutant, p777, accumulated 8% more oil in seed than did wild type, but it showed no differences in seed size, plant growth or development. The high-oil phenotype is caused by the disruption of the GLABRA2 gene, a previously identified gene that encodes a homeobox protein required for normal trichome and root hair development. Knockout of GLABRA2 did not affect LEAFY COTYLEDON 1 and PICKLE expression in developing embryo. The result indicates that in addition to its known function in trichome and root hair development, GLABRA2 is involved in the control of seed oil accumulation. Springer, 2006 density screen nationallicence GLABRA 2 nationallicence high oil nationallicence mucilage nationallicence trichome nationallicence Shen Bo Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut Sinkevicius Kerstin Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut Selinger David Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut Tarczynski Mitchell Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 377-387 0167-4412 60:3<377 2006 60 11103 https://doi.org/10.1007/s11103-005-4110-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4110-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Bo Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut NATIONALLICENCE 700 1- Sinkevicius Kerstin Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut NATIONALLICENCE 700 1- Selinger David Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut NATIONALLICENCE 700 1- Tarczynski Mitchell Pioneer Hi-Bred International, a DuPont Company, 7300 NW 62nd Ave, 50131, Johnston, Iowa, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 377-387 0167-4412 60:3<377 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer