caa a22 4500 467893683 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4209-4 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4209-4 Transcription Profile Analyses Identify Genes and Pathways Central to Root Cap Functions in Maize [Elektronische Daten] [Keni Jiang, Shibo Zhang, Stanley Lee, George Tsai, Kyungpil Kim, Haiyan Huang, Charles Chilcott, Tong Zhu, Lewis Feldman] Affymetrix GeneChips arrayed with about one-half (~23K) of the rice genes were used to profile gene transcription activity in three tissues comprising the maize root tip; the proximal meristem (PM), the quiescent center (QC), and the root cap (RC). Here we analyze the gene transcription profile of the RC, compared to both the PM and the QC, from three biological replicates. In the RC, a total of 669 genes were identified as being differentially upregulated, and 365 differentially downregulated. Real-time quantitative RT-PCR analysis was used to confirm upregulated genes in the RC. In addition, using the technique of laser microdissection (LMD) we localized upregulated gene expression to the lateral RC cells. Taken as a whole, transcription profile analyses revealed the upregulation in the maize RC of clusters of genes linked to major metabolic processes and pathways, including: (1) transport, both the export of carbohydrates and the uptake of nutrients; (2) sensing and responding to (often stressful) biotic and abiotic environmental stimuli; (3) integrating the responses of at least 3 major growth regulators (auxin, ethylene, jasmonic acid); (4) processing the large amount of carbohydrate transported into the RC. Although the profile data are derived using heterologous rice GeneChips, with about half of the total rice gene set, this study, nevertheless, provides a genomic scale characterization of the entire RC, and serves as a new platform from which to advance studies of the network of pathways operating in the maize RC. Springer, 2006 maize nationallicence microarray nationallicence root cap nationallicence transcription profile nationallicence Jiang Keni Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut Zhang Shibo Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut Lee Stanley Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut Tsai George Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut Kim Kyungpil Department of Statistics, University of California, 94720, Berkeley, CA, USA aut Huang Haiyan Department of Statistics, University of California, 94720, Berkeley, CA, USA aut Chilcott Charles Syngenta Biotechnology, Inc., 3054 Cornwallis Road, 27709, Research Triangle Park, NC, USA aut Zhu Tong Syngenta Biotechnology, Inc., 3054 Cornwallis Road, 27709, Research Triangle Park, NC, USA aut Feldman Lewis Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 343-363 0167-4412 60:3<343 2006 60 11103 https://doi.org/10.1007/s11103-005-4209-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4209-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Keni Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Zhang Shibo Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Lee Stanley Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Tsai George Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Kim Kyungpil Department of Statistics, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Huang Haiyan Department of Statistics, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 700 1- Chilcott Charles Syngenta Biotechnology, Inc., 3054 Cornwallis Road, 27709, Research Triangle Park, NC, USA aut NATIONALLICENCE 700 1- Zhu Tong Syngenta Biotechnology, Inc., 3054 Cornwallis Road, 27709, Research Triangle Park, NC, USA aut NATIONALLICENCE 700 1- Feldman Lewis Department of Plant and Microbial Biology, University of California, 94720, Berkeley, CA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 343-363 0167-4412 60:3<343 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer