caa a22 4500 467893691 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4211-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-4211-x The Early Dark-Response in Arabidopsis thaliana Revealed by cDNA Microarray Analysis [Elektronische Daten] [Byung-Hoon Kim, Albrecht von Arnim] Despite intense research on light responses in plants, the consequences of a simple shift from light to darkness remain poorly characterized. We have examined the transcriptome of Arabidopsis thaliana seedling leaves upon a shift from constant light to darkness for between 1 and 8h, while excluding most effects associated with circadian oscillation. Expression clustering and gene ontology analyses identified about 790 responsive genes implicated in diverse cellular processes. Compared to the better-studied long-term dark adaptation response, the early response to darkness is partially overlapping yet clearly distinct, encompassing early transient, early sustained, and late response clusters. The repressor of photomorphogenesis, COP1 (constitutive photomorphogenic 1), is not a chief regulator of the early response to darkness, in contrast to its well-established role during long-term dark adaptation and etiolation. Only part of the early dark response can be understood as the opposite of the response following a dark-to-light transition and as a response to sugar deprivation. Bioinformatic comparisons with published microarray datasets further suggest that abscisic acid (ABA) signaling plays a prominent role in the early response to darkness, although this effect is not mediated by an increase in the ABA level. The potential basis for the co-regulation by darkness and ABA is discussed in light of sugar and redox signaling. Springer, 2006 abscisic acid nationallicence Arabidopsis nationallicence dark nationallicence microarray nationallicence photomorphogenesis nationallicence starvation nationallicence ABA : abscisic acid nationallicence ATH1 : Arabidopsis thaliana homeobox 1 nationallicence BL : brassinolide nationallicence CAB2 : chlorophyll a/b-binding protein 2 nationallicence CCA1 : circadian clock associated 1 nationallicence COP : constitutive photomorphogenic nationallicence cry : cryptochrome nationallicence CSN : COP9 signalosome nationallicence DET1 : de-etiolated 1 nationallicence DIG : digoxigenin nationallicence EF1-α : translation elongation factor 1-α nationallicence FEDA : ferredoxin A nationallicence GA : gibberellic acid nationallicence GO : gene ontology nationallicence HY5 : long hypocotyl 5 nationallicence IAA : indole-acetic acid nationallicence pgm : phosphoglucomutase nationallicence phot : phototropin nationallicence phy : phytochrome nationallicence RT-PCR : reverse transcription polymerase chain reaction nationallicence SEN : senescence associated nationallicence TCH4 : touch 4 nationallicence TIR1 : transport inhibitor response nationallicence Kim Byung-Hoon Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, 37996-0840, Knoxville, TN, USA aut von Arnim Albrecht Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, 37996-0840, Knoxville, TN, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 321-342 0167-4412 60:3<321 2006 60 11103 https://doi.org/10.1007/s11103-005-4211-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4211-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Byung-Hoon Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, 37996-0840, Knoxville, TN, USA aut NATIONALLICENCE 700 1- von Arnim Albrecht Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, 37996-0840, Knoxville, TN, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 321-342 0167-4412 60:3<321 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer