caa a22 4500 467893705 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4770-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-4770-x Dual Function of Rice OsDR8 Gene in Disease Resistance and Thiamine Accumulation [Elektronische Daten] [Gongnan Wang, Xinhua Ding, Meng Yuan, Deyun Qiu, Xianghua Li, Caiguo Xu, Shiping Wang] The function of OsDR8, a rice disease resistance-responsive gene, was studied. Silencing of OsDR8 using an RNA interference approach resulted in phenotypic alteration of the plants. The transgenic plants with repressed expression of OsDR8 showed reduced resistance or susceptibility to Xanthomonas oryzae pv. oryzae and Magnaporthe grisea causing bacterial blight and blast, which are two of the most devastating diseases in rice worldwide, respectively. The putative product of OsDR8 was highly homologous to an enzyme involved in the biosynthesis of the thiazole precursor of thiamine. Transgenic plants showing repressed expression of OsDR8 and reduced resistance had significantly lower levels of thiamine than the control plants. Exogenous application of thiamine could complement the compromised defense of the OsDR8-silenced plants. The expression level of several defense-responsive genes including the earlier functional genes of defense transduction pathway, OsPOX and OsPAL, and the downstream genes of the pathway, OsPR1a, OsPR1b, OsPR4, OsPR5 and OsPR10, was also decreased in the OsDR8-silenced plants. These results suggest that the impact of OsDR8 on disease resistance in rice may be through the regulation of expression of other defense-responsive genes and the site of OsDR8 function is on the upstream of the signal transduction pathway. In addition, the accumulation of thiamine may be essential for bacterial blight resistance and blast resistance. Springer, 2006 bacterial blight nationallicence blast nationallicence defense nationallicence rice nationallicence thiamine nationallicence PR : pathogenesis-related nationallicence QTLs : quantitative trait loci nationallicence R : resistance gene nationallicence RACE : rapid amplification of cDNA end nationallicence RNAi : RNA interference nationallicence RT : reverse transcription nationallicence SA : salicylic acid nationallicence UTR : untranslated region nationallicence Xoo : Xanthomonas oryzae pv. Oryzae nationallicence Wang Gongnan National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Ding Xinhua National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Yuan Meng National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Qiu Deyun National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Li Xianghua National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Xu Caiguo National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Wang Shiping National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 437-449 0167-4412 60:3<437 2006 60 11103 https://doi.org/10.1007/s11103-005-4770-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4770-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Gongnan National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Ding Xinhua National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Yuan Meng National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Qiu Deyun National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Li Xianghua National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Xu Caiguo National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Wang Shiping National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 437-449 0167-4412 60:3<437 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer