caa a22 4500 467893713 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s11103-005-4477-z doi (NATIONALLICENCE)springer-10.1007/s11103-005-4477-z Regulatory Sequences of Orthologous petD Chloroplast mRNAs are Highly Specific among Chlamydomonas Species [Elektronische Daten] [Lynn Kramzar, Toby Mueller, Brian Erickson, David Higgs] The 5′ untranslated regions (UTR) of chloroplast mRNAs often contain regulatory sequences that control RNA stability and/or translation. The petD chloroplast mRNA in Chlamydomonas reinhardtii has three such essential regulatory elements in its 362-nt long 5′ UTR. To further analyze these elements, we compared 5′ UTR sequences from four Chlamydomonas species (C. reinhardtii, C. incerta, C. moewusii and C. eugametos) and five independent strains of C. reinhardtii. Overall, these petD 5′ UTRs have relatively low sequence conservation across these species. In contrast, sequences of the three regulatory elements and their relative positions appear partially conserved. Functionality of the 5′ UTRs was tested in C. reinhardtii chloroplasts using β-glucuronidase reporter genes, and the nearly identical C. incerta petD functioned for mRNA stability and translation in C. reinhardtii chloroplasts while the more divergent C. eugametos petD did not. This identified what may be key features in these elements. We conclude that these petD regulatory elements, and possibly the corresponding trans-acting factors, function via mechanisms highly specific and surprisingly sensitive to minor sequence changes. This provides a new and broader perspective of these important regulatory sequences that affect photosynthesis in these algae. Springer, 2006 organelles nationallicence photosynthesis nationallicence phylogenetics nationallicence plastids nationallicence post-transcription nationallicence Kramzar Lynn Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut Mueller Toby Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut Erickson Brian Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut Higgs David Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 405-422 0167-4412 60:3<405 2006 60 11103 https://doi.org/10.1007/s11103-005-4477-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4477-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kramzar Lynn Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut NATIONALLICENCE 700 1- Mueller Toby Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut NATIONALLICENCE 700 1- Erickson Brian Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut NATIONALLICENCE 700 1- Higgs David Department of Biological Sciences, University of Wisconsin-Parkside, 53141, Kenosha, WI, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/3(2006-02-01), 405-422 0167-4412 60:3<405 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer