caa a22 4500 467893721 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5257-5 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5257-5 An Auxin-Inducible F-Box Protein CEGENDUO Negatively Regulates Auxin-Mediated Lateral Root Formation in Arabidopsis [Elektronische Daten] [Li Dong, Lei Wang, Yu'e Zhang, Yansheng Zhang, Xingwang Deng, Yongbiao Xue] Previously, we characterized 92 Arabidopsis genes (AtSFLs) similar to the S-locus F-box genes involved in S-RNase-based self-incompatibility and found that they likely play diverse roles in Arabidopsis. In this study, we investigated the role of one of these genes, CEGENDUO (CEG, AtSFL61), in the lateral root formation. A T-DNA insertion in CEG led to an increased lateral root production, which was complemented by transformation of the wild-type gene. Its downregulation by RNAi also produced more lateral roots in transformed Arabidopsis plants whereas its overexpression generated less lateral roots compared to wild-type, indicating that CEG acts as a negative regulator for the lateral root formation. It was found that CEG was expressed abundantly in vascular tissues of the primary root, but not in newly formed lateral root primordia and the root meristem, and induced by exogenous auxin NAA (α-naphthalene acetic acid). In addition, the ceg mutant was hyposensitive to NAA, IAA (indole-3-acetic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid), as well as the auxin transport inhibitor TIBA (3,3,5-triiodobenzoic acid), showing that CEG is an auxin-inducible gene. Taken together, our results show that CEG is a novel F-box protein negatively regulating the auxin-mediated lateral root formation in Arabidopsis. Springer, 2006 Arabidopsis nationallicence auxin nationallicence F-box protein nationallicence lateral root formation nationallicence Dong Li Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut Wang Lei Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut Zhang Yu'e Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut Zhang Yansheng Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut Deng Xingwang Department of Molecular, Cellular, and Developmental Biology, Yale University, 06520-8104, New Haven, CT, USA aut Xue Yongbiao Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 599-615 0167-4412 60:4<599 2006 60 11103 https://doi.org/10.1007/s11103-005-5257-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5257-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Li Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut NATIONALLICENCE 700 1- Wang Lei Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut NATIONALLICENCE 700 1- Zhang Yu'e Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut NATIONALLICENCE 700 1- Zhang Yansheng Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut NATIONALLICENCE 700 1- Deng Xingwang Department of Molecular, Cellular, and Developmental Biology, Yale University, 06520-8104, New Haven, CT, USA aut NATIONALLICENCE 700 1- Xue Yongbiao Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, 100080, Beijing, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 599-615 0167-4412 60:4<599 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer