caa a22 4500 46789373X CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-4922-z doi (NATIONALLICENCE)springer-10.1007/s11103-005-4922-z The Rice OsRad21-4, an Orthologue of Yeast Rec8 Protein, is Required for Efficient Meiosis [Elektronische Daten] [Liangran Zhang, Jiayi Tao, Shunxin Wang, Kang Chong, Tai Wang] In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes that share lower than 21% identity at polypeptide levels, and each is present as a single copy in this genome. Here we describe our analysis of the function of OsRAD21-4 by RNAi. Western blot analyses indicated that the protein was most abundant in young flowers and less in leaves and buds but absent in roots. In flowers, the expression was further defined to premeiotic pollen mother cells (PMCs) and meiotic PMCs of anthers. Meiotic chromosome behaviors were monitored from male meiocytes of OsRAD21-4-deficient lines mediated by RNAi. The male meiocytes showed multiple aberrant events at meiotic prophase I, including over-condensation of chromosomes, precocious segregation of homologues and chromosome fragmentation. Fluorescence in situ hybridization experiments revealed that the deficient lines were defective in homologous pairing and cohesion at sister chromatid arms. These defects resulted in unequal chromosome segregation and aberrant spore generation. These observations suggest that OsRad21-4 is essential for efficient meiosis. Springer, 2006 cohesion nationallicence condensation nationallicence meiosis nationallicence OsRAD21-4 nationallicence Oryza sativa nationallicence pairing nationallicence Zhang Liangran Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Tao Jiayi Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Wang Shunxin Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Chong Kang Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Wang Tai Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 533-554 0167-4412 60:4<533 2006 60 11103 https://doi.org/10.1007/s11103-005-4922-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4922-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Liangran Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Tao Jiayi Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Wang Shunxin Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Chong Kang Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Wang Tai Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Research Center of Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 533-554 0167-4412 60:4<533 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer