caa a22 4500 467893748 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5082-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-5082-x Expression analysis of the AtMLO Gene Family Encoding Plant-Specific Seven-Transmembrane Domain Proteins [Elektronische Daten] [Zhongying Chen, H. Hartmann, Ming-Jing Wu, Erin Friedman, Jin-Gui Chen, Matthew Pulley, Paul Schulze-Lefert, Ralph Panstruga, Alan Jones] The Arabidopsis (Arabidopsis thaliana) genome contains 15 genes encoding protein homologs of the barley mildew resistance locus o (MLO) protein biochemically shown to have a seven-transmembrane domain topology and localize to the plasma membrane. Towards elucidating the functions of MLOs, the largest family of seven-transmembrane domain proteins specific to plants, we comprehensively determined AtMLO gene expression patterns by a combination of experimental and in silico studies. Experimentation comprised analyses of transgenic Arabidopsis lines bearing promoter::β-glucuronidase (GUS) transcriptional fusions as well as semi-quantitative determination of transcripts by reverse transcription coupled to polymerase chain reaction (RT-PCR). These results were combined with information extracted from public gene profiling databases, and compared to the expression patterns of genes encoding the heterotrimeric G-protein subunits. We found that each AtMLO gene has a unique expression pattern and is regulated differently by a variety of biotic and/or abiotic stimuli, suggesting that AtMLO proteins function in diverse developmental and response processes. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s). Springer, 2006 Arabidopsis thaliana nationallicence GUS staining nationallicence heterotrimeric G-protein nationallicence microarray nationallicence MLO nationallicence RT-PCR nationallicence AGB1 : the Arabidopsis Gβ subunit nationallicence AGG1 : the Arabidopsis Gγ subunit1 nationallicence AGG2 : the Arabidopsis Gγ subunit2 nationallicence FRET : Förster/Fluorescence Resonance Energy Transfer nationallicence GCR1 : G-Coupled Receptor1 nationallicence GPA1 : the Arabidopsis Gα subunit nationallicence GPCRs : G-protein coupled receptors nationallicence GUS : β-glucuronidase nationallicence MLO : mildew resistance locus o nationallicence RGS1 : Regulator of G-protein Signaling nationallicence 7TM : seven-transmembrane nationallicence Chen Zhongying Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut Hartmann H. Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Wu Ming-Jing Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut Friedman Erin Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut Chen Jin-Gui Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut Pulley Matthew Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut Schulze-Lefert Paul Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Panstruga Ralph Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Jones Alan Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 583-597 0167-4412 60:4<583 2006 60 11103 https://doi.org/10.1007/s11103-005-5082-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5082-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Zhongying Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Hartmann H. Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 700 1- Wu Ming-Jing Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Friedman Erin Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Chen Jin-Gui Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Pulley Matthew Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Schulze-Lefert Paul Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 700 1- Panstruga Ralph Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 700 1- Jones Alan Department of Biology, The University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 583-597 0167-4412 60:4<583 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer