caa a22 4500 467893756 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-4844-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4844-9 Expression of Bacterial l -aspartate-α-decarboxylase in Tobacco Increases β-Alanine and Pantothenate Levels and Improves Thermotolerance [Elektronische Daten] [Walid Fouad, Bala Rathinasabapathi] l-Aspartate-α-decarboxylase catalyzes the decarboxylation of l-aspartate to generate β-alanine and carbon dioxide. This is an unusual pyruvoyl-dependent enzyme unique to prokaryotes that undergoes limited self-processing. The Escherichia coli panD gene encoding l-aspartate-α-decarboxylase was expressed under a constitutive promoter in transgenic tobacco. Transgene expression was verified by assays based on RNA blots, immunoblots and enzyme activity in vitro. The panD lines had increased levels of leaf β-alanine (1.2- to 4-fold), pantothenate (3.2- to 4.1-fold) and total free amino acids (up to 3.7-fold) compared to wild-type and vector controls. Growth of homozygous lines expressing E. coli l-aspartate-α-decarboxylase was less affected than that of the control lines when the plants were stressed for 1 week at 35 °C. When transferred from 35 to 30 °C for 3 weeks, the PanD transgenic lines recovered significantly (P ≤ 0.001) better than the controls: PanD lines had on an average 54% and 84% greater fresh and dry weights respectively, compared to the controls. Homozygous lines expressing E. coli l-aspartate-α-decarboxylase had significantly greater thermotolerance (P ≤ 0.05) during germination. At 42 °C, 95% of two T3 PanD transgenic line seeds germinated after 12 days compared to 73% for the wild-type seeds. Our results indicated that E. colil-aspartate-α-decarboxylase was correctly processed and active in the transgenic eukaryotic host and its expression resulted in increased thermotolerance in tobacco. Springer, 2006 l- aspartate-α-decarboxylase nationallicence β-alanine nationallicence panD nationallicence seed germination nationallicence thermotolerance nationallicence Fouad Walid Plant Molecular and Cellular Biology Program, Horticultural Sciences Department, University of Florida, Hull Road, 1301 Fifield Hall, P.O. Box 110 690, 32611-0690, Gainesville, FL, USA aut Rathinasabapathi Bala Plant Molecular and Cellular Biology Program, Horticultural Sciences Department, University of Florida, Hull Road, 1301 Fifield Hall, P.O. Box 110 690, 32611-0690, Gainesville, FL, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 495-505 0167-4412 60:4<495 2006 60 11103 https://doi.org/10.1007/s11103-005-4844-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4844-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fouad Walid Plant Molecular and Cellular Biology Program, Horticultural Sciences Department, University of Florida, Hull Road, 1301 Fifield Hall, P.O. Box 110 690, 32611-0690, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Rathinasabapathi Bala Plant Molecular and Cellular Biology Program, Horticultural Sciences Department, University of Florida, Hull Road, 1301 Fifield Hall, P.O. Box 110 690, 32611-0690, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 495-505 0167-4412 60:4<495 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer