caa a22 4500 467893772 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-4924-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-4924-x Generation of Phenylpropanoid Pathway-Derived Volatiles in Transgenic Plants: Rose Alcohol Acetyltransferase Produces Phenylethyl Acetate and Benzyl Acetate in Petunia Flowers [Elektronische Daten] [Inna Guterman, Tania Masci, Xinlu Chen, Florence Negre, Eran Pichersky, Natalia Dudareva, David Weiss, Alexander Vainstein] Esters are important contributors to the aroma of numerous flowers and fruits. Acetate esters such as geranyl acetate, phenylethyl acetate and benzyl acetate are generated as a result of the action of alcohol acetyltransferases (AATs). Numerous homologous AATs from various plants have been characterized using in-vitro assays. To study the function of rose alcohol acetyltransferase (RhAAT) in planta, we generated transgenic petunia plants expressing the rose gene under the control of a CaMV-35S promoter. Although the preferred substrate of RhAAT in vitro is geraniol, in transgenic petunia flowers, it used phenylethyl alcohol and benzyl alcohol to produce the corresponding acetate esters, not generated by control flowers. The level of benzyl alcohol emitted by the flowers of different transgenic lines was ca. three times higher than that of phenylethyl alcohol, which corresponded to the ratio between the respective products, i.e. ca. three times more benzyl acetate than phenylethyl acetate. Feeding of transgenic petunia tissues with geraniol or octanol led to the production of their respective acetates, suggesting the dependence of volatile production on substrate availability. Springer, 2006 alcohol acetyltransferases nationallicence benzenoid/phenylpropanoid pathway nationallicence flower fragrance nationallicence metabolic engineering nationallicence volatile esters nationallicence Guterman Inna The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut Masci Tania The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut Chen Xinlu Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907-2010, Indiana, USA aut Negre Florence Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907-2010, Indiana, USA aut Pichersky Eran Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, 48109, Michigan, USA aut Dudareva Natalia Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907-2010, Indiana, USA aut Weiss David The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut Vainstein Alexander The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 555-563 0167-4412 60:4<555 2006 60 11103 https://doi.org/10.1007/s11103-005-4924-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4924-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guterman Inna The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Masci Tania The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Chen Xinlu Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907-2010, Indiana, USA aut NATIONALLICENCE 700 1- Negre Florence Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907-2010, Indiana, USA aut NATIONALLICENCE 700 1- Pichersky Eran Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, 48109, Michigan, USA aut NATIONALLICENCE 700 1- Dudareva Natalia Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, 47907-2010, Indiana, USA aut NATIONALLICENCE 700 1- Weiss David The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Vainstein Alexander The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 555-563 0167-4412 60:4<555 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer