caa a22 4500 467893799 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5022-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5022-9 Wood Formation from the Base to the Crown in Pinus Radiata [Elektronische Daten] Gradients of Tracheid Wall Thickness, Wood Density, Radial Growth Rate and Gene Expression [Sheree Cato, Lisa McMillan, Lloyd Donaldson, Thomas Richardson, Craig Echt, Richard Gardner] Wood formation was investigated at five heights along the bole for two unrelated trees of Pinus radiata. Both trees showed clear gradients in wood properties from the base to the crown. Cambial cells at the base of the tree were dividing 3.3-fold slower than those at the crown, while the average thickness of cell walls in wood was highest at the base. Cell wall thickness showed an overall correlation coefficient of >0.7 with wood density in both genotypes. Microscopic examination of developing tracheids showed that 33% of cells had formed secondary cell walls at the base of the tree, reducing to 3% at the crown. In total, 455 genes differentially expressed in developing xylem tissue from either the base or the crown were identified using modified differential display. RT-PCR analysis of 156 genes confirmed differential expression for 77%. Of the genes tested, 73% showed gradients in transcript abundance either up or down the bole of the tree, although the steepness of the gradients differed between genes. Genes involved in cell division and expansion tended to be more highly expressed in the crown of the tree, and two putative cell-cycle repressor genes were expressed 2-fold higher at the base. Conversely, transcripts of genes involved in secondary wall thickening were more abundant at the base of the tree. These results suggest that differences in the rate of cambial cell division, differences in the rate and duration of tracheid wall thickening, and differences in gene expression underpin the gradients of wood properties found in pines. Springer, 2006 gene expression nationallicence Pinus radiata nationallicence secondary cell wall nationallicence wall thickness nationallicence wood density nationallicence xylem nationallicence Cato Sheree Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut McMillan Lisa Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut Donaldson Lloyd Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut Richardson Thomas Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut Echt Craig Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut Gardner Richard School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 565-581 0167-4412 60:4<565 2006 60 11103 https://doi.org/10.1007/s11103-005-5022-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5022-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cato Sheree Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- McMillan Lisa Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Donaldson Lloyd Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Richardson Thomas Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Echt Craig Scion (formerly Forest Research), Cellwall Biotechnology Centre, 49 Sala Street, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Gardner Richard School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 565-581 0167-4412 60:4<565 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer