caa a22 4500 467893802 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-4921-0 doi (NATIONALLICENCE)springer-10.1007/s11103-005-4921-0 Characterization of the Snowy Cotyledon 1 Mutant of Arabidopsis Thaliana [Elektronische Daten] The Impact of Chloroplast Elongation Factor G on Chloroplast Development and Plant Vitality [Verónica Albrecht, Anke Ingenfeld, Klaus Apel] During seedling development chloroplast formation marks the transition from heterotrophic to autotrophic growth. The development and activity of chloroplasts may differ in cotyledons that initially serve as a storage organ and true leaves whose primary function is photosynthesis. A genetic screen was used for the identification of genes that affect selectively chloroplast function in cotyledons of Arabidopsis thaliana. Several mutants exhibiting pale cotyledons and green true leaves were isolated and dubbed snowy cotyledon (sco).One of the mutants, sco1, was characterized in more detail. The mutated gene was identified using map-based cloning. The mutant contains a point mutation in a gene encoding the chloroplast elongation factor G, leading to an amino acid exchange within the predicted 70S ribosome-binding domain. The mutation results in a delay in the onset of germination. At this early developmental stage embryos still contain undifferentiated proplastids, whose proper function seems necessary for seed germination. In light-grown sco1 seedlings the greening of cotyledons is severely impaired, whereas the following true leaves develop normally as in wild-type plants. Despite this apparent similarity of chloroplast development in true leaves of mutant and wild-type plants various aspects of mature plant development are also affected by the sco1 mutation such as the onset of flowering, the growth rate, and seed production. The onset of senescence in the mutant and the wild-type plants occurs, however, at the same time, suggesting that in the mutant this particular developmental step does not seem to suffer from reduced protein translation efficiency in chloroplasts. Springer, 2006 chloroplast development nationallicence chloroplast protein biosynthesis nationallicence snowy cotyledon 1 nationallicence plant vitality nationallicence Albrecht Verónica Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland aut Ingenfeld Anke Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland aut Apel Klaus Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland aut Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 507-518 0167-4412 60:4<507 2006 60 11103 https://doi.org/10.1007/s11103-005-4921-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-4921-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Albrecht Verónica Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland aut NATIONALLICENCE 700 1- Ingenfeld Anke Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland aut NATIONALLICENCE 700 1- Apel Klaus Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/4(2006-03-01), 507-518 0167-4412 60:4<507 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer