caa a22 4500 467893829 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5750-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-5750-x The Heat Stress Transcription Factor HsfA2 Serves as a Regulatory Amplifier of a Subset of Genes in the Heat Stress Response in Arabidopsis [Elektronische Daten] [Franziska Schramm, Arnab Ganguli, Elke Kiehlmann, Gisela Englich, Daniela Walch, Pascal von Koskull-Döring] Within the Arabidopsis family of 21 heat stress transcription factors (Hsfs) HsfA2 is the strongest expressed member under heat stress (hs) conditions. Irrespective of the tissue, HsfA2 accumulates under heat stress similarly to other heat stress proteins (Hsps). A SALK T-DNA insertion line with a complete HsfA2-knockout was analyzed with respect to the changes in the transcriptome under heat stress conditions. Ascorbate peroxidase 2 (APX2) was identified as the most affected transcript in addition to several sHsps, individual members of the Hsp70 and Hsp100 family, as well as many transcripts of genes with yet unknown functions. For functional validation, the transcription activation potential of HsfA2 on GUS reporter constructs containing 1kb upstream promoter sequences of selected target genes were analyzed using transient reporter assays in mesophyll protoplasts. By deletion analysis the promoter region of the strongest affected target gene APX2 was functionally mapped in detail to verify potential HsfA2 binding sites. By electrophoretic mobility shift assays we identified TATA-Box proximal clusters of heat stress elements (HSE) in the promoters of selected target genes as potential HsfA2 binding sites. The results presented here demonstrate that the expression of HsfA2 in Arabidopsis is strictly heat stress-dependent and this transcription factor represents a regulator of a subset of stress response genes in Arabidopsis. Springer, 2006 Arabidopsis nationallicence heat stress nationallicence HsfA2 knockout nationallicence target genes nationallicence transcription factor nationallicence APX2 : ascorbate peroxidase 2 nationallicence CTD : C-terminal domain nationallicence DBD : DNA-binding domain nationallicence EMSA : electrophoretic mobility shift assay nationallicence GUS : β-glucuronidase nationallicence hs : heat stress nationallicence HSE : heat stress element nationallicence Hsf : heat stress transcription factor nationallicence Hsp : heat stress protein nationallicence ko : knockout nationallicence wt : wild type nationallicence Schramm Franziska Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut Ganguli Arnab Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut Kiehlmann Elke Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut Englich Gisela Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut Walch Daniela Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut von Koskull-Döring Pascal Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 759-772 0167-4412 60:5<759 2006 60 11103 https://doi.org/10.1007/s11103-005-5750-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5750-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schramm Franziska Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut NATIONALLICENCE 700 1- Ganguli Arnab Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut NATIONALLICENCE 700 1- Kiehlmann Elke Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut NATIONALLICENCE 700 1- Englich Gisela Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut NATIONALLICENCE 700 1- Walch Daniela Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut NATIONALLICENCE 700 1- von Koskull-Döring Pascal Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 759-772 0167-4412 60:5<759 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer