caa a22 4500 467893837 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5620-6 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5620-6 Rejuvenation by Shoot Apex Culture Recapitulates the Developmental Increase of Methylation at the Maize Gene Pl-Blotched [Elektronische Daten] [Erin Irish, Douglas McMurray] Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a myb-like transcription factor that regulates anthocyanin production in maize, is one such gene: certain cytosines at the 3′ end of this allele are hypomethylated in seedlings, become hypermethylated in organs formed in the adult phase, and are hypomethylated again in the next generation. We tested whether this developmental pattern of low juvenile cytosine methylation followed by higher methylation in adult tissues could also be observed in plants "rejuvenated” via shoot apex culture. We found that cytosine methylation patterns at Pl-Blotched were indeed recapitulated in culture-rejuvenated plants, showing hypomethylation in leaves with juvenile patterns of differentiation (even though they were made by an old meristem) followed by hypermethylation in later-formed leaves. Our results show that methylation status at that locus is determined by the developmental phase of the shoot, rather than by the age of the meristem forming it. These results support the hypothesis that DNA methylation is employed by the plant to maintain an epigenetic state. Springer, 2006 cytosine methylation nationallicence epigenetic nationallicence phase change nationallicence plant development nationallicence Irish Erin Department of Biological Sciences, The University of Iowa, 52242, Iowa City, IA, USA aut McMurray Douglas Department of Biological Sciences, The University of Iowa, 52242, Iowa City, IA, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 747-758 0167-4412 60:5<747 2006 60 11103 https://doi.org/10.1007/s11103-005-5620-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5620-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Irish Erin Department of Biological Sciences, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 700 1- McMurray Douglas Department of Biological Sciences, The University of Iowa, 52242, Iowa City, IA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 747-758 0167-4412 60:5<747 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer