caa a22 4500 467893853 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5547-y doi (NATIONALLICENCE)springer-10.1007/s11103-005-5547-y Expression of a Brassica Isopropylmalate Synthase Gene in Arabidopsis Perturbs Both Glucosinolate and Amino Acid Metabolism [Elektronische Daten] [Ben Field, Caroline Furniss, Andrew Wilkinson, Richard Mithen] Isopropylmalate synthase (IPMS) is a key enzyme in the biosynthesis of the essential amino acid leucine, and thus primary metabolism. In Arabidopsis, the functionally similar enzyme, methythiolalkylmalate synthase (MAM), is an important enzyme in the elongation of methionine prior to glucosinolate (GSL) biosynthesis, as part of secondary metabolism. We describe the cloning of an IPMS gene from Brassica, BatIMS, and its functional characterisation by heterologous expression in E. coli and Arabidopsis. Over expression of BatIMS in Arabidopsis resulted in plants with an aberrant phenotype, reminiscent of mutants in GSL biosynthesis. Metabolite analyses showed that these plants had both perturbed amino acid metabolism and enhanced levels of GSLs. Microarray profiling showed that BatIMS over expression caused up regulation of the genes for methionine-derived GSL biosynthesis, and down regulation of genes involved in leucine catabolism, in addition to perturbed expression of genes involved in auxin and ethylene metabolism. The results illustrate the cross talk that can occur between primary and secondary metabolism within transgenic plants. Springer, 2006 Arabidopsis nationallicence Brassica nationallicence glucosinolate nationallicence IPMS nationallicence isopropylmalate synthase nationallicence leucine nationallicence Field Ben John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut Furniss Caroline Phytochemicals and Health Programme, Institute of Food Research, NR4 7UA, Norwich, UK aut Wilkinson Andrew Phytochemicals and Health Programme, Institute of Food Research, NR4 7UA, Norwich, UK aut Mithen Richard Phytochemicals and Health Programme, Institute of Food Research, NR4 7UA, Norwich, UK aut Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 717-727 0167-4412 60:5<717 2006 60 11103 https://doi.org/10.1007/s11103-005-5547-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5547-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Field Ben John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK aut NATIONALLICENCE 700 1- Furniss Caroline Phytochemicals and Health Programme, Institute of Food Research, NR4 7UA, Norwich, UK aut NATIONALLICENCE 700 1- Wilkinson Andrew Phytochemicals and Health Programme, Institute of Food Research, NR4 7UA, Norwich, UK aut NATIONALLICENCE 700 1- Mithen Richard Phytochemicals and Health Programme, Institute of Food Research, NR4 7UA, Norwich, UK aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 717-727 0167-4412 60:5<717 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer