caa a22 4500 467893861 CHVBK 20180406152756.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5441-7 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5441-7 Expression Profiles of 10,422 Genes at Early Stage of Low Nitrogen Stress in Rice Assayed using a cDNA Microarray [Elektronische Daten] [Xingming Lian, Shiping Wang, Jianwei Zhang, Qi Feng, Lida Zhang, Danlin Fan, Xianghua Li, Dejun Yuan, Bin Han, Qifa Zhang] Development of crop varieties with high nitrogen use efficiency (NUE) is imperative for sustainable agriculture. Understanding how plant genes respond to low N stress is essential for formulating approaches to manipulating genes for improving NUE. In this study we analyzed the expression profiles of an indica rice cultivar Minghui 63 at seedling stage at 20min, 1 and 2h after low N stress with the normal N as the control, using a microarray of 11,494 rice ESTs representing 10,422 unique genes. While no significant difference was detected in the leaf tissue, a total of 471 ESTs were detected as responsive to low N stress in the root tissue with 115 ESTs showing up-regulation and 358 ESTs showing down-regulation. The analysis of expression profiles after low N stress identified following patterns: (1) the genes involved in photosynthesis and energy metabolism were down-regulated rapidly; (2) many of the genes involved in early responses to biotic and abiotic stresses were up-regulated while many other stress responsive genes were down-regulated; (3) regulatory genes including transcription factors and ones involved in signal transduction were both up- and down-regulated; and (4) the genes known to be involved in N uptake and assimilation showed little response to the low N stress. The challenges for future studies are to characterize the functional roles of the low N stress responsive genes in N metabolisms, including the large number of genes presently with unknown functions. Springer, 2006 cDNA chip nationallicence ESTs nationallicence nitrogen use efficiency (NUE) nationallicence Oryza sativa L nationallicence Lian Xingming National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Wang Shiping National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Zhang Jianwei National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Feng Qi National Center for Gene Research, Chinese Academy of Sciences, 200233, Shanghai, China aut Zhang Lida National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Fan Danlin National Center for Gene Research, Chinese Academy of Sciences, 200233, Shanghai, China aut Li Xianghua National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Yuan Dejun National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Han Bin National Center for Gene Research, Chinese Academy of Sciences, 200233, Shanghai, China aut Zhang Qifa National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 617-631 0167-4412 60:5<617 2006 60 11103 https://doi.org/10.1007/s11103-005-5441-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5441-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lian Xingming National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Wang Shiping National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Jianwei National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Feng Qi National Center for Gene Research, Chinese Academy of Sciences, 200233, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Lida National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Fan Danlin National Center for Gene Research, Chinese Academy of Sciences, 200233, Shanghai, China aut NATIONALLICENCE 700 1- Li Xianghua National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Yuan Dejun National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Han Bin National Center for Gene Research, Chinese Academy of Sciences, 200233, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Qifa National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 617-631 0167-4412 60:5<617 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer