caa a22 4500 467893896 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5546-z doi (NATIONALLICENCE)springer-10.1007/s11103-005-5546-z The A and B Loci in Tobacco Regulate a Network of Stress Response Genes, Few of which are Associated with Nicotine Biosynthesis [Elektronische Daten] [Sarah Kidd, Amanda Melillo, Rong-He Lu, Deborah Reed, Norihito Kuno, Kenko Uchida, Masaki Furuya, John Jelesko] Nicotine biosynthesis in Nicotianatabacum is under genetic control by the A and B loci. Plants containing semi-dominant mutations at both the A and B loci (i.e. aabb genotype) have lower nicotine levels, reduced nicotine biosynthetic enzyme activities, and reduced mRNA levels of the corresponding biosynthetic genes. The A and B loci therefore appear to be coordinate regulators of several nicotine biosynthetic genes and define a group of co-regulated genes called the A-B regulon. To investigate the composition of genes in the A-B regulon, a fluorescent differential display (FDD) screen was used to randomly sample the transcriptomes of wild type and mutant aabb roots. This resulted in the isolation of 64 FDD clones, representing 49 unique genes or gene families. Four genes associated with nicotine biosynthesis were identified, whereas most of the other FDD clones were homologous with an assortment of stress response genes. Thirty-three genes or gene families showed reproducible aabb genotype effects, representing seven distinct mRNA expression patterns in response media treatments that increase the mRNA levels of known alkaloid biosynthetic genes. Thus, the A and B loci regulate the mRNA levels of some target genes differently than others. Eleven genes or gene families showed only treatment-specific effects, representing four mRNA accumulation patterns. These results indicate the A-B regulon is complex network of differentially expressed stress response genes, only a small subset of which are involved in nicotine biosynthesis. Springer, 2006 A and B loci nationallicence alkaloid nationallicence fluorescent differential display nationallicence NIC1 nationallicence NIC2 nationallicence Nicotiana tabacum nationallicence nicotine nationallicence transcript profiling nationallicence tobacco nationallicence ADC : arginine decarboxylase nationallicence B21 : Burley 21 nationallicence dAO : Diamine oxidase nationallicence FDD : fluorescent differential display nationallicence LA21 : low alkaloid Burley 21 nationallicence IBA : indolebutyric acid nationallicence ODC : ornithine decarboxylase nationallicence MJA : methyljasmonic acid nationallicence PMT : putrescine methyltransferase nationallicence QS : quinolinate synthetase nationallicence QPT : quinolinate phosphoribosyltransferase nationallicence SAMS : S-adenosylmethionine synthetase nationallicence STR : strictosidine synthase nationallicence TIA : terpene indole alkaloids nationallicence Kidd Sarah Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut Melillo Amanda Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut Lu Rong-He Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut Reed Deborah Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut Kuno Norihito Advanced Research Laboratories, Hitachi, 350-0395, Hatoyama, Saitama, Japan aut Uchida Kenko Advanced Research Laboratories, Hitachi, 350-0395, Hatoyama, Saitama, Japan aut Furuya Masaki Advanced Research Laboratories, Hitachi, 350-0395, Hatoyama, Saitama, Japan aut Jelesko John Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 699-716 0167-4412 60:5<699 2006 60 11103 https://doi.org/10.1007/s11103-005-5546-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5546-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kidd Sarah Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut NATIONALLICENCE 700 1- Melillo Amanda Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut NATIONALLICENCE 700 1- Lu Rong-He Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut NATIONALLICENCE 700 1- Reed Deborah Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut NATIONALLICENCE 700 1- Kuno Norihito Advanced Research Laboratories, Hitachi, 350-0395, Hatoyama, Saitama, Japan aut NATIONALLICENCE 700 1- Uchida Kenko Advanced Research Laboratories, Hitachi, 350-0395, Hatoyama, Saitama, Japan aut NATIONALLICENCE 700 1- Furuya Masaki Advanced Research Laboratories, Hitachi, 350-0395, Hatoyama, Saitama, Japan aut NATIONALLICENCE 700 1- Jelesko John Plant Pathology, Physiology, and Weed Science Department, Virginia Polytechnic Institute and State University, 24061-0346, Blacksburg, Virginia, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 699-716 0167-4412 60:5<699 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer