caa a22 4500 46789390X CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5472-0 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5472-0 "HAIRY CANOLA” - Arabidopsis GL3 Induces a Dense Covering of Trichomes on Brassica napus Seedlings [Elektronische Daten] [M. Gruber, S. Wang, S. Ethier, J. Holowachuk, P. Bonham-Smith, J. Soroka, A. Lloyd] Transformation with the Arabidopsis bHLH gene 35S:GLABRA3 (GL3) produced novel B. napus plants with an extremely dense coverage of trichomes on seedling tissues (stems and young leaves). In contrast, trichomes were strongly induced in seedling stems and moderately induced in leaves of a hairy, purple phenotype transformed with a 2.2kb allele of the maize anthocyanin regulator LEAF COLOUR (Lc), but only weakly induced by BOOSTER (B-Peru), the maize Lc 2.4kb allele, or the Arabidopsis trichome MYB gene GLABRA1 (GL1). B. napus plants containing only the GL3 transgene had a greater proportion of trichomes on the adaxial leaf surface, whereas all other plant types had a greater proportion on the abaxial surface. Progeny of crosses between GL3+ and GL1+ plants resulted in trichome densities intermediate between a single-insertion GL3+ plant and a double-insertion GL3+ plant. None of the transformations stimulated trichomes on Brassica cotyledons or on non-seedling tissues. A small portion of bHLH gene-induced trichomes had a swollen terminal structure. The results suggest that trichome development in B. napus may be regulated differently from Arabidopsis. They also imply that insertion of GL3 into Brassica species under a tissue-specific promoter has strong potential for developing insect-resistant crop plants. Springer, 2006 Brassica napus nationallicence Lc nationallicence B-Peru nationallicence C1 nationallicence GL3 nationallicence GL1 nationallicence MYB nationallicence bHLH regulatory transgenes nationallicence plant transformation nationallicence trichome stimulation nationallicence Gruber M. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut Wang S. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut Ethier S. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut Holowachuk J. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut Bonham-Smith P. Department of Biology, University of Saskatchewan, 112 Science Place, SK S7N 5E2, Saskatoon, Canada aut Soroka J. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut Lloyd A. Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, TX 78713, Austin, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 679-698 0167-4412 60:5<679 2006 60 11103 https://doi.org/10.1007/s11103-005-5472-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5472-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gruber M. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Wang S. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Ethier S. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Holowachuk J. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Bonham-Smith P. Department of Biology, University of Saskatchewan, 112 Science Place, SK S7N 5E2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Soroka J. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, SK S7N 0X2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Lloyd A. Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, TX 78713, Austin, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/5(2006-03-01), 679-698 0167-4412 60:5<679 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer