caa a22 4500 467893918 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s11103-005-5091-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5091-9 Asparagales Telomerases which Synthesize the Human Type of Telomeres [Elektronische Daten] [Eva Sýkorová, Andrew Leitch, Jiří Fajkus] The order of monocotyledonous plants Asparagales is attractive for studies of telomere evolution as it includes three phylogenetically distinct groups with telomeres composed of TTTAGGG (Arabidopsis-type), TTAGGG (human-type) and unknown alternative sequences, respectively. To analyze the molecular causes of these switches in telomere sequence (synthesis), genes coding for the catalytic telomerase subunit (TERT) of representative species in the first two groups have been cloned. Multiple alignments of the sequences, together with other TERT sequences in databases, suggested candidate amino acid substitutions grouped in the Asparagales TERT synthesizing the human-type repeat that could have contributed to the changed telomere sequence. Among these, mutations in the C motif are of special interest due to its functional importance in TERT. Furthermore, two different modes of initial elongation of the substrate primer were observed in Asparagales telomerases producing human-like repeats, which could be attributed to interactions between the telomerase RNA subunit (TR) and the substrate. Springer, 2006 Asparagales nationallicence evolution nationallicence telomerase nationallicence template region nationallicence Sýkorová Eva Laboratory of DNA-Molecular Complexes, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-61265, Brno, Czech Republic aut Leitch Andrew School of Biological Sciences, Queen Mary University of London, Mile End Road, E1 4NS, London, UK aut Fajkus Jiří Laboratory of DNA-Molecular Complexes, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-61265, Brno, Czech Republic aut Plant Molecular Biology Springer Netherlands 60/5(2006-03-01), 633-646 0167-4412 60:5<633 2006 60 11103 https://doi.org/10.1007/s11103-005-5091-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5091-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sýkorová Eva Laboratory of DNA-Molecular Complexes, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-61265, Brno, Czech Republic aut NATIONALLICENCE 700 1- Leitch Andrew School of Biological Sciences, Queen Mary University of London, Mile End Road, E1 4NS, London, UK aut NATIONALLICENCE 700 1- Fajkus Jiří Laboratory of DNA-Molecular Complexes, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-61265, Brno, Czech Republic aut NATIONALLICENCE 773 0- Plant Molecular Biology Springer Netherlands 60/5(2006-03-01), 633-646 0167-4412 60:5<633 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer