caa a22 4500 467893934 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-3880-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-3880-9 AtSAP18, An Orthologue of Human SAP18, is Involved in the Regulation of Salt Stress and Mediates Transcriptional Repression in Arabidopsis [Elektronische Daten] [Chun-Peng Song, David Galbraith] In yeast and mammalian systems, it is well established that transcriptional down-regulation by DNA-binding repressors involves core histone deacetylation, mediated by their interaction within a complex containing histone deacetylase (e.g. HDA1), as well as various other proteins (e.g. SIN3, SAP18, SAP30, and RbAp46). Here we identify that a Arabidopsis thaliana gene related in sequence to SAP18, designated AtSAP18, functions in transcription regulation in plants subjected to salt stress. The AtSAP18 loss- of-function mutant is more sensitive to NaCl, and is impaired in chlorophyll synthesis as compared to the wild-type. Using GST pull-down, two-hybrid, and transient transcription assays, we have characterized SAP18 and HDA1 orthologues and provide evidence that SAP18 and HDA1 function as transcriptional repressors. We further demonstrate that they associate with Ethylene-Responsive Element binding Factors (ERFs) to create a hormone-sensitive multimeric repressor complex under conditions of environmental stress. Our results indicate that AtSAP18 functions to link the HDA complex to transcriptional repressors that are bound to chromatin in a sequence-specific manner, thereby providing the specificity of signal transduction accompanying transcriptional repression under stress conditions. Springer, 2006 Arabidopsis thaliana nationallicence histone deacetylation nationallicence stress nationallicence transcriptional repression nationallicence HDA : histone deacetylase nationallicence Song Chun-Peng Department of Plant Sciences, University of Arizona, 303 Forbes Building, 85721, Tucson, AZ, USA aut Galbraith David Department of Plant Sciences, University of Arizona, 303 Forbes Building, 85721, Tucson, AZ, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 241-257 0167-4412 60:2<241 2006 60 11103 https://doi.org/10.1007/s11103-005-3880-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-3880-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Chun-Peng Department of Plant Sciences, University of Arizona, 303 Forbes Building, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Galbraith David Department of Plant Sciences, University of Arizona, 303 Forbes Building, 85721, Tucson, AZ, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 241-257 0167-4412 60:2<241 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer