caa a22 4500 467893950 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-3568-1 doi (NATIONALLICENCE)springer-10.1007/s11103-005-3568-1 Comparative Structural and Functional Characterization of Sorghum and Maize Duplications Containing Orthologous Myb Transcription Regulators of 3-Deoxyflavonoid Biosynthesis [Elektronische Daten] [Jayanand Boddu, Cizhong Jiang, Vineet Sangar, Terry Olson, Thomas Peterson, Surinder Chopra] Sequence characterization of the genomic region of sorghum yellow seed1 shows the presence of two genes that are arranged in a head to tail orientation. The two duplicated gene copies, y1 and y2 are separated by a 9.084kbp intergenic region, which is largely composed of highly repetitive sequences. The y1 is the functional copy, while the y2 may represent a pseudogene; there are several sequence indels and rearrangements within the putative coding region of y2. The y1 gene encodes a R2R3 type of Myb domain protein that regulates the expression of chalcone synthase, chalcone isomerase and dihydroflavonol reductase genes required for the biosynthesis of 3-deoxyflavonoids. Expression of y1 can be observed throughout the plant and it represents a combination of expression patterns produced by different alleles of the maize p1. Comparative sequence analysis within the coding regions and flanking sequences of y1, y2 and their maize and teosinte orthologs show local rearrangements and insertions that may have created modified regulatory regions. These micro-colinearity modifications possibly are responsible for differential patterns of expression in maize and sorghum floral and vegetative tissues. Phylogenetic analysis indicates that sorghum y1 and y2 sequences may have arisen by gene duplication mechanisms and represent an evolutionarily parallel event to the duplication of maize p2 and p1 genes. Springer, 2006 3-deoxyflavonoids nationallicence anthocyanins nationallicence flavonoid nationallicence gene structure nationallicence sorghum nationallicence maize nationallicence Myb nationallicence pericarp nationallicence phlobaphenes nationallicence phytoalexins nationallicence Boddu Jayanand Department of Crop & Soil Sciences, Pennsylvania State University, 16802, PA, USA aut Jiang Cizhong Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut Sangar Vineet Department of Crop & Soil Sciences, Pennsylvania State University, 16802, PA, USA aut Olson Terry Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut Peterson Thomas Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut Chopra Surinder Department of Crop & Soil Sciences, Pennsylvania State University, 16802, PA, USA aut Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 185-199 0167-4412 60:2<185 2006 60 11103 https://doi.org/10.1007/s11103-005-3568-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-3568-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boddu Jayanand Department of Crop & Soil Sciences, Pennsylvania State University, 16802, PA, USA aut NATIONALLICENCE 700 1- Jiang Cizhong Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Sangar Vineet Department of Crop & Soil Sciences, Pennsylvania State University, 16802, PA, USA aut NATIONALLICENCE 700 1- Olson Terry Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Peterson Thomas Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Chopra Surinder Department of Crop & Soil Sciences, Pennsylvania State University, 16802, PA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 185-199 0167-4412 60:2<185 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer