caa a22 4500 467893977 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-3129-7 doi (NATIONALLICENCE)springer-10.1007/s11103-005-3129-7 Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza virus Confers Resistance to the Virus in Transgenic Mexican Lime [Elektronische Daten] [Carmen Fagoaga, Carmelo López, Alfonso de Mendoza, Pedro Moreno, Luis Navarro, Ricardo Flores, Leandro Peña] Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific small interfering RNAs (siRNAs). When graft- or aphid-inoculated with CTV, some propagations of these silenced lines were immune: they neither expressed symptoms nor accumulated virions and viral RNA as estimated by DAS-ELISA and Northern blot hybridization, respectively. Other propagations were moderately resistant because they became infected later and showed attenuated symptoms compared to controls. The susceptible propagations, in addition to symptom expression and elevated virus titer, accumulated p23-specific siRNAs at levels significantly higher than immune or non-inoculated propagations, and showed transgene demethylation. This variable response among clonal transformants indicates that factors other than the genetic background of the transgenic plants play a key role in PTGS-mediated resistance. Springer, 2006 CTV nationallicence methylation nationallicence pathogenicity determinant nationallicence RNA silencing nationallicence small interfering RNAs nationallicence virus resistance nationallicence Fagoaga Carmen Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut López Carmelo Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC) Universidad Politécnica de Valencia, Avenida de los Naranjos, 46022, Valencia, Spain aut de Mendoza Alfonso Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut Moreno Pedro Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut Navarro Luis Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut Flores Ricardo Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC) Universidad Politécnica de Valencia, Avenida de los Naranjos, 46022, Valencia, Spain aut Peña Leandro Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 153-165 0167-4412 60:2<153 2006 60 11103 https://doi.org/10.1007/s11103-005-3129-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-3129-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fagoaga Carmen Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut NATIONALLICENCE 700 1- López Carmelo Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC) Universidad Politécnica de Valencia, Avenida de los Naranjos, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- de Mendoza Alfonso Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut NATIONALLICENCE 700 1- Moreno Pedro Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut NATIONALLICENCE 700 1- Navarro Luis Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut NATIONALLICENCE 700 1- Flores Ricardo Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC) Universidad Politécnica de Valencia, Avenida de los Naranjos, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Peña Leandro Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada, 46113, Valencia, Spain aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 153-165 0167-4412 60:2<153 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer