caa a22 4500 467894000 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-3688-7 doi (NATIONALLICENCE)springer-10.1007/s11103-005-3688-7 Ordered Assembly of Mitochondria During Rice Germination Begins with Promitochondrial Structures Rich in Components of the Protein Import Apparatus [Elektronische Daten] [Katharine Howell, A. Millar, James Whelan] Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound promitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48h following imbibition, an ordered increase in the abundance of transcripts encoding mitochondrial proteins was observed. Co-incident with these changes in transcript levels was dynamic and rapid changes in mitochondrial protein content and mitochondrial function. Proteins representing components of the mitochondrial protein import apparatus are strikingly abundant in dry seeds, and a functional import apparatus was shown to operate 2h after imbibition. Interestingly, this import process was best driven by the oxidation of NADH from outside the mitochondrial inner membrane. In later developmental stages the capacity for matrix organic acid metabolism was evident, accompanied by the appearance of proteins for TCA cycle components, and coordination of electron transport chain assembly through components encoded in both mitochondrial and nuclear genomes. Together these events provide new insights into the understanding of mitochondrial maturation and the nature of promitochondrial structures in plant cells. Springer, 2006 mitochondrial biogenesis nationallicence promitochondria nationallicence protein import nationallicence germination nationallicence ANT : adenine nucleotide translocator nationallicence Aox : alternative oxidase nationallicence HSP : heat shock protein nationallicence LEA : late embryogenesis abundant nationallicence OGDC : oxoglutarate dehydrogenase complex nationallicence PDC : pyruvate dehydrogenase complex nationallicence qRT-PCR : quantitative reverse transcription PCR nationallicence RISP : Rieske iron-sulfur protein nationallicence TCA : tricarboxylic acid nationallicence TIM : translocase of the inner membrane nationallicence TOM : translocase of the outer membrane nationallicence UCP : uncoupling protein nationallicence Howell Katharine ARC Centre of Excellence in Plant Energy Biology, CMS Building M310, University of Western Australia, 35 Stirling Hwy, 6009, Perth, WA, Australia aut Millar A. ARC Centre of Excellence in Plant Energy Biology, CMS Building M310, University of Western Australia, 35 Stirling Hwy, 6009, Perth, WA, Australia aut Whelan James ARC Centre of Excellence in Plant Energy Biology, CMS Building M310, University of Western Australia, 35 Stirling Hwy, 6009, Perth, WA, Australia aut Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 201-223 0167-4412 60:2<201 2006 60 11103 https://doi.org/10.1007/s11103-005-3688-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-3688-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Howell Katharine ARC Centre of Excellence in Plant Energy Biology, CMS Building M310, University of Western Australia, 35 Stirling Hwy, 6009, Perth, WA, Australia aut NATIONALLICENCE 700 1- Millar A. ARC Centre of Excellence in Plant Energy Biology, CMS Building M310, University of Western Australia, 35 Stirling Hwy, 6009, Perth, WA, Australia aut NATIONALLICENCE 700 1- Whelan James ARC Centre of Excellence in Plant Energy Biology, CMS Building M310, University of Western Australia, 35 Stirling Hwy, 6009, Perth, WA, Australia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 201-223 0167-4412 60:2<201 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer