caa a22 4500 467894019 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s11103-005-3381-x doi (NATIONALLICENCE)springer-10.1007/s11103-005-3381-x Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray* [Elektronische Daten] [Fanchang Zeng, Xianlong Zhang, Longfu Zhu, Lili Tu, Xiaoping Guo, Yichun Nie] Somatic embryogenesis (SE) is the developmental reprogramming of somatic cells toward the embryogenesis pathway and is a notable illustration of cell totipotency. To identify genes involved in SE, subtractive polymerase chain reaction (PCR) was performed to generate transcripts highly enriched for SE-related genes, using cDNA prepared from a mixture of embryogenic callus and preglobular somatic embryos, as the tester, and cDNA from nonembryogenic callus, as the driver. After differential screening and subsequent confirmation by reverse Northern blot analysis, a total of 671 differentially expressed cDNA fragments were identified, and 242 unigenes significantly up-regulated during cotton SE were recovered, as confirmed by Northern blot and reverse-transcription PCR analysis of representative cases, including most previously published SE-related genes in plants. In total, more than half had not been identified previously as SE-related genes, including dominant crucial genes involved in transcription, posttranscription, and transportation, and about one-third had not been reported previously to GenBank or were expected to be unknown, or newly identified genes. We used cDNA arrays to further investigate the expression patterns of these genes in differentiating gradient culture, ranging from proembryogenic masses to somatic embryos at every stage. The cDNA collection is composed of a broad repertoire of SE genes which is an important resource for understanding the genetic interactions underlying SE signaling and regulation. Our results suggested that a complicated and concerted mechanism involving multiple cellular pathways is responsible for cotton SE. This report represents a systematic and comprehensive analysis of genes involved in the process of somatic embryogenesis. Springer, 2006 cotton nationallicence cDNA arrays nationallicence somatic embryogenesis nationallicence suppression subtractive hybridization nationallicence transcriptome analysis nationallicence CE : cotyledonary-stage embryo nationallicence EC : embryogenic callus nationallicence EST : expressed sequence tag nationallicence GE : globular embryo nationallicence GO : gene ontology nationallicence HE : heart embryo nationallicence LTP : lipid-transfer protein nationallicence NEC : nonembryogenic callus nationallicence PEC : primary embryogenic callus nationallicence PEM : proembryogenic masses nationallicence PGE : preglobular embryo nationallicence PPR : pentatricopeptide repeat nationallicence RT-PCR : reverse-transcription-polymerase chain reaction nationallicence SCR : SCARECROW nationallicence SE : somatic embryogenesis nationallicence SSH : suppression subtractive hybridization nationallicence TE : torpedo embryo nationallicence ZF-HD : zinc finger homeodomain nationallicence Zeng Fanchang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut Zhang Xianlong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut Zhu Longfu National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut Tu Lili National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut Guo Xiaoping National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut Nie Yichun National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 167-183 0167-4412 60:2<167 2006 60 11103 https://doi.org/10.1007/s11103-005-3381-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-3381-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng Fanchang National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut NATIONALLICENCE 700 1- Zhang Xianlong National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut NATIONALLICENCE 700 1- Zhu Longfu National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut NATIONALLICENCE 700 1- Tu Lili National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut NATIONALLICENCE 700 1- Guo Xiaoping National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut NATIONALLICENCE 700 1- Nie Yichun National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P. R. China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 60/2(2006-01-01), 167-183 0167-4412 60:2<167 2006 60 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer