caa a22 4500 467894027 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0032-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0032-9 Suppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele in Brassica self-incompatibility [Elektronische Daten] [Ryo Fujimoto, Tetsu Sugimura, Eigo Fukai, Takeshi Nishio] Mutations in the S locus of a self-compatible cultivar Yellow Sarson in Brassica rapa, which has a self-compatible class-I S haplotype, S-f2, were investigated. S-28 in Brassica oleracea was found to be a member of an interspecific pair with S-f2 in B. rapa. The original S haplotype of S-f2 was identified to be S-54 in B. rapa. Sequence comparison of alleles in S-f2 with those in S-54 and B. oleracea S-28 revealed insertion of a retrotransposon-like sequence in the first intron of SRK and 89-bp deletion in the promoter region of SP11. No transcripts of SRK and SP11 were detected in S-f2 homozygotes, suggesting that the insertion and the deletion in SRK and SP11, respectively, caused the loss of the function of these genes. Promoter assay using transgenic plants indicated that the SP11 promoter of S-f2 has no activity. Heterozygotes of S-f2 and a normal class-II S haplotype, S-60, in B. rapa were found to be self-compatible. Interestingly, transcription of SP11-60 was revealed to be suppressed in the S-f2/S-60 heterozygotes, suggesting that an untranscribed class-I SP11 allele suppresses the expression of a recessive class-II SP11 allele in the anthers of S heterozygotes. Similar phenomenon was observed in heterozygotes of a self-compatible class-I S haplotype and a self-incompatible class-II S haplotype in B. oleracea. Springer Science+Business Media B.V., 2006 Dominant/recessive relationship nationallicence Gene expression nationallicence Mutation nationallicence Retrotransposon nationallicence S locus nationallicence Self-incompatibility nationallicence BoS-28 : S-28 in Brassica oleracea nationallicence BrS-f2 : S-f2 in Brassica rapa nationallicence BrSP11-f2 : SP11 of BrS-f2 nationallicence BrSRK-f2 : SRK of BrS-f2 nationallicence BrSTFf2a : B. rapa S locus retrotransposon family-f2a nationallicence FLC : FLOWERING LOCUS C nationallicence GUS : β-glucuronidase nationallicence SCR : S-locus cysteine rich protein nationallicence SLG : S-locus glycoprotein nationallicence SP11 : S-locus protein 11 nationallicence SRK : S-locus receptor kinase nationallicence Fujimoto Ryo Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut Sugimura Tetsu Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut Fukai Eigo Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut Nishio Takeshi Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 577-587 0167-4412 61:4-5<577 2006 61 11103 https://doi.org/10.1007/s11103-006-0032-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0032-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujimoto Ryo Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Sugimura Tetsu Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Fukai Eigo Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 700 1- Nishio Takeshi Graduate School of Agricultural Science, Tohoku University, 981-8555, Aoba-ku, Sendai, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 577-587 0167-4412 61:4-5<577 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer