caa a22 4500 467894043 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0035-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0035-6 Targeted Ds -tagging strategy generates high allelic diversity at the Arabidopsis HY2 locus [Elektronische Daten] [Audrey Creff, Bénédicte Léonard, Thierry Desnos] The targeted (or directed) tagging is a strategy aimed to mobilize a tranposon into a specific gene (target). Only a very few Arabidopsis genes have been tagged by this way, thus the efficiency of the strategy, as well as the diversity of the alleles obtained are not well documented. We have used a maize Ds element in a directed tagging of HY2. The starting Ds element, located 22kb proximal to HY2, has been remobilized in a cross with an Ac transposase source line. From the F2 progeny of 4800 F1 we phenotypically isolated seven hy2 mutants. Molecular analysis of these alleles revealed that two contained a Ds element in HY2 and were instable, three have a large deletion that partially or completely removed HY2, one has a footprint in a HY2 exon and one leaky allele consisted of a 22kb inversion upstream the HY2 coding sequence. Thus, the transposon-based directed tagging strategy generates a wide diversity of tagged and non-tagged alleles that can be used to generate allelic series or deletion of clustered genes. Springer Science+Business Media B.V., 2006 Transposons nationallicence Ac/Ds nationallicence Targeted tagging nationallicence Arabidopsis nationallicence hy2 nationallicence GUS : β-glucuronidase nationallicence Creff Audrey CEA cadarache, DSV/DEVM Laboratoire de Biologie du Développement des Plantes, Bât. 178, UMR6191, CEA/CNRS/Université Aix-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France aut Léonard Bénédicte CEA cadarache, DSV/DEVM Laboratoire de Biologie du Développement des Plantes, Bât. 178, UMR6191, CEA/CNRS/Université Aix-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France aut Desnos Thierry CEA cadarache, DSV/DEVM Laboratoire de Biologie du Développement des Plantes, Bât. 178, UMR6191, CEA/CNRS/Université Aix-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 603-613 0167-4412 61:4-5<603 2006 61 11103 https://doi.org/10.1007/s11103-006-0035-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0035-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Creff Audrey CEA cadarache, DSV/DEVM Laboratoire de Biologie du Développement des Plantes, Bât. 178, UMR6191, CEA/CNRS/Université Aix-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France aut NATIONALLICENCE 700 1- Léonard Bénédicte CEA cadarache, DSV/DEVM Laboratoire de Biologie du Développement des Plantes, Bât. 178, UMR6191, CEA/CNRS/Université Aix-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France aut NATIONALLICENCE 700 1- Desnos Thierry CEA cadarache, DSV/DEVM Laboratoire de Biologie du Développement des Plantes, Bât. 178, UMR6191, CEA/CNRS/Université Aix-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 603-613 0167-4412 61:4-5<603 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer