caa a22 4500 46789406X CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0051-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0051-6 The 73 kD Subunit of the cleavage and polyadenylation specificity factor (CPSF) complex affects reproductive development in Arabidopsis [Elektronische Daten] [Ruqiang Xu, Hongwei Zhao, Randy Dinkins, Xiaowen Cheng, George Carberry, Qingshun Li] The cleavage and polyadenylation specificity factor (CPSF) is an important multi-subunit component of the mRNA 3′-end processing apparatus in eukaryotes. The Arabidopsis genome contains five genes encoding CPSF homologues (AtCPSF160, AtCPSF100, AtCPSF73-I, AtCPSF73-II and AtCPSF30). These CPSF homologues interact with each other in a way that is analogous to the mammalian CPSF complex or their yeast counterparts, and also interact with the Arabidopsis poly(A) polymerase (PAP). There are two CPSF73 like proteins (AtCPSF73-I and AtCPSF73-II) that share homology with the 73 kD subunit of the mammalian CPSF complex. AtCPSF73-I appears to correspond to the functionally characterized mammalian CPSF73 and its yeast counterpart. AtCPSF73-II was identified as a novel protein with uncharacterized protein homologues in other multicellular organisms, but not in yeast. Both of the AtCPSF73 proteins are targeted in the nucleus and were found to interact with AtCPSF100. They are also essential since knockout or knockdown mutants are lethal. In addition, the expression level of AtCPSF73-I is critical for Arabidopsis development because overexpression of AtCPSF73-I is lethal. Interestingly, transgenic plants carrying an additional copy of the AtCPSF73-I gene, that is, the full-length cDNA under the control of its native promoter, appeared normal but were male sterile due to delayed anther dehiscence. In contrast, we previously demonstrated that a mutation in the AtCPSF73-II gene was detrimental to the genetic transmission of female gametes. Thus, two 73 kD subunits of the AtCPSF complex appear to have special functions during flower development. The important roles of mRNA 3′-end processing machinery in modulating plant development are discussed. Springer Science+Business Media B.V., 2006 mRNA 3′-end processing nationallicence Polyadenylation nationallicence CPSF complex nationallicence Male sterility nationallicence AD : activation domain nationallicence BD : DNA binding domain nationallicence CF : cleavage factor nationallicence CPSF : cleavage and polyadenylation specificity factor nationallicence CstF : cleavage stimulatory factor nationallicence CTD : C-terminal domain nationallicence DEX : dexamethasone nationallicence GFP : green fluorescence protein nationallicence GST : glutathione transferase nationallicence GUS : β-glucuronidase nationallicence PAP : poly(A) polymerase nationallicence RNAi : RNA interference nationallicence RNAP II : RNA polymerase II nationallicence Xu Ruqiang Department of Botany, Miami University, 45056, Oxford, OH, USA aut Zhao Hongwei Department of Botany, Miami University, 45056, Oxford, OH, USA aut Dinkins Randy USDA-ARS Forage-Animal Production Research Unit, KY40546, Lexington, USA aut Cheng Xiaowen Department of Microbiology, Miami University, 45056, Oxford, OH, USA aut Carberry George Department of Botany, Miami University, 45056, Oxford, OH, USA aut Li Qingshun Department of Botany, Miami University, 45056, Oxford, OH, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 799-815 0167-4412 61:4-5<799 2006 61 11103 https://doi.org/10.1007/s11103-006-0051-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0051-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Ruqiang Department of Botany, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Zhao Hongwei Department of Botany, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Dinkins Randy USDA-ARS Forage-Animal Production Research Unit, KY40546, Lexington, USA aut NATIONALLICENCE 700 1- Cheng Xiaowen Department of Microbiology, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Carberry George Department of Botany, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Li Qingshun Department of Botany, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 799-815 0167-4412 61:4-5<799 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer