caa a22 4500 467894078 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0040-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0040-9 Two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics [Elektronische Daten] [Daniel Manríquez, Islam El-Sharkawy, Francisco Flores, Fikri El-Yahyaoui, Farid Regad, Mondher Bouzayen, Alain Latché, Jean-Claude Pech] Alcohol dehydrogenases (ADH) participate in the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. Two highly divergent ADH genes (15% identity at the amino acid level) of Cantaloupe Charentais melon (Cucumis melo var. Cantalupensis) have been isolated. Cm-ADH1 belongs to the medium-chain zinc-binding type of ADHs and is highly similar to all ADH genes expressed in fruit isolated so far. Cm-ADH2 belongs to the short-chain type of ADHs. The two encoded proteins are enzymatically active upon expression in yeast. Cm-ADH1 has strong preference for NAPDH as a co-factor, whereas Cm-ADH2 preferentially uses NADH. Both Cm-ADH proteins are much more active as reductases with K ms 10-20 times lower for the conversion of aldehydes to alcohols than for the dehydrogenation of alcohols to aldehydes. They both show strong preference for aliphatic aldehydes but Cm-ADH1 is capable of reducing branched aldehydes such as 3-methylbutyraldehyde, whereas Cm-ADH2 cannot. Both Cm-ADH genes are expressed specifically in fruit and up-regulated during ripening. Gene expression as well as total ADH activity are strongly inhibited in antisense ACC oxidase melons and in melon fruit treated with the ethylene antagonist 1-methylcyclopropene (1-MCP), indicating a positive regulation by ethylene. These data suggest that each of the Cm-ADH protein plays a specific role in the regulation of aroma biosynthesis in melon fruit. Springer Science+Business Media B.V., 2006 Alcohol dehydrogenase/aldehyde reductase nationallicence Aroma volatiles nationallicence Ethylene nationallicence Fruit ripening nationallicence Medium- and short-chain ADH nationallicence Melon nationallicence Manríquez Daniel UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut El-Sharkawy Islam UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut Flores Francisco UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut El-Yahyaoui Fikri UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut Regad Farid UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut Bouzayen Mondher UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut Latché Alain UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut Pech Jean-Claude UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 675-685 0167-4412 61:4-5<675 2006 61 11103 https://doi.org/10.1007/s11103-006-0040-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0040-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manríquez Daniel UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- El-Sharkawy Islam UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- Flores Francisco UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- El-Yahyaoui Fikri UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- Regad Farid UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- Bouzayen Mondher UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- Latché Alain UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 700 1- Pech Jean-Claude UMR 990 INRA/INPT-ENSAT "Génomique et Biotechnologie des Fruits”, Av. de l'Agrobiopole, BP 32607, F-31326, Castanet-Tolosan Cedex, France aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 675-685 0167-4412 61:4-5<675 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer