caa a22 4500 467894086 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0049-0 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0049-0 Floral patterning defects induced by Arabidopsis APETALA2 and microRNA172 expression in Nicotiana benthamiana [Elektronische Daten] [Sizolwenkosi Mlotshwa, Zhiyong Yang, YunJu Kim, Xuemei Chen] Floral patterning and morphogenesis are controlled by many transcription factors including floral homeotic proteins, by which floral organ identity is determined. Recent studies have uncovered widespread regulation of transcription factors by microRNAs (miRNAs), ~21-nucleotide non-coding RNAs that regulate protein-coding RNAs through transcript cleavage and/or translational inhibition. The regulation of the floral homeotic gene APETALA2 (AP2) by miR172 is crucial for normal Arabidopsis flower development and is likely to be conserved across plant species. Here we probe the activity of the AP2/miR172 regulatory circuit in a heterologous Solanaceae species, Nicotiana benthamiana. We generated transgenic N. benthamiana lines expressing Arabidopsis wild type AP2 (35S::AP2), miR172-resistant AP2 mutant (35S::AP2m3) and MIR172a-1 (35S::MIR172) under the control of the cauliflower mosaic virus 35S promoter. 35S::AP2m3 plants accumulated high levels of AP2 mRNA and protein and exhibited floral patterning defects that included proliferation of numerous petals, stamens and carpels indicating loss of floral determinacy. On the other hand, nearly all 35S::AP2 plants accumulated barely detectable levels of AP2 mRNA or protein and were essentially non-phenotypic. Overall, the data indicated that expression of the wild type Arabidopsis AP2 transgene was repressed at the mRNA level by an endogenous N. benthamiana miR172 homologue that could be detected using Arabidopsis miR172 probe. Interestingly, 35S::MIR172 plants had sepal-to-petal transformations and/or more sepals and petals, suggesting interference with N. benthamiana normal floral homeotic gene function in perianth organs. Our studies uncover the potential utility of the Arabidopsis AP2/miR172 system as a tool for manipulation of floral architecture and flowering time in non-model plants. Springer Science+Business Media B.V., 2006 APETALA2 nationallicence Arabidopsis nationallicence microRNA nationallicence miR172 nationallicence Nicotiana benthamiana nationallicence AG : AGAMOUS nationallicence AP2 : APETALA2 nationallicence CaMV : cauliflower mosaic virus nationallicence DEF : DEFICIENS nationallicence GLO : GLOBOSA nationallicence HMW RNA : high molecular weight RNA nationallicence LMW RNA : low molecular weight RNA nationallicence miRNA : microRNA nationallicence miR172 : microRNA172 nationallicence NAG1 : Nicotiana tabacum AGAMOUS nationallicence NbAG : Nicotiana benthamiana AGAMOUS nationallicence NbAP2 : Nicotiana benthamiana APETALA2 nationallicence NbDEF : Nicotiana benthamiana DEFICIENS nationallicence NbGLO : Nicotiana benthamiana GLOBOSA nationallicence NbWUS : Nicotiana benthamiana WUSCHEL nationallicence RUBISCO : ribulose bisphosphate carboxylase nationallicence TOE : TARGET OF EAT nationallicence WUS : WUSCHEL nationallicence Mlotshwa Sizolwenkosi Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut Yang Zhiyong Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut Kim YunJu Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut Chen Xuemei Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 781-793 0167-4412 61:4-5<781 2006 61 11103 https://doi.org/10.1007/s11103-006-0049-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0049-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mlotshwa Sizolwenkosi Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut NATIONALLICENCE 700 1- Yang Zhiyong Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut NATIONALLICENCE 700 1- Kim YunJu Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut NATIONALLICENCE 700 1- Chen Xuemei Department of Botany & Plant Sciences, University of California, 92521, California, Riverside, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 781-793 0167-4412 61:4-5<781 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer