caa a22 4500 467894094 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0048-1 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0048-1 A single hemoglobin gene in Myrica gale retains both symbiotic and non-symbiotic specificity [Elektronische Daten] [Anne Heckmann, Kim Hebelstrup, Knud Larsen, Nuno Micaelo, Erik Jensen] Here, a hemoglobin gene from the nitrogen-fixing actinorhizal plant Myrica gale was isolated, cloned and sequenced. The gene (MgHb) was a class I hemoglobin with strong sequence homology to non-symbiotic hemoglobin genes. MgHb is highly expressed in symbiotic root nodules, but transcripts and protein were also detected in leaves of M. gale. In Arabidopsis thaliana the MgHb promoter, linked to a β-glucuronidase coding region, directed expression in the vascular tissue, in shoot meristem and at root branch point - a pattern very similar to the combined expression pattern of the two non-symbiotic A. thaliana hemoglobin promoters AHb1 and AHb2. The results points to a symbiotic as well as a non-symbiotic specificity of MgHb similar to a hemoglobin gene identified in Parasponia andersonii, but different from the situation in Casuarina glauca - a close actinorhizal relative of M. gale. Springer Science+Business Media B.V., 2006 Evolution nationallicence Hemoglobin nationallicence Myrica gale nationallicence Symbiotic nationallicence ACC : 1-Aminocyclopropane-1-carboxyl acid nationallicence GUS : ß-Glucuronidase nationallicence MgHb : Myrica gale hemoglobin nationallicence WT : Wildtype nationallicence Heckmann Anne Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus Gustav Wieds Vej, 10C DK-8000, Aarhus C, Denmark aut Hebelstrup Kim Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus Gustav Wieds Vej, 10C DK-8000, Aarhus C, Denmark aut Larsen Knud Department of Crop Physiology and Soil Science, Danish Institute of Agricultural Science, 50, DK-8830, Tjele, Denmark aut Micaelo Nuno Institutode Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, EAN, ITQB II, Piso 6, Apartado 127, 2781-901, Oeiras, Portugal aut Jensen Erik Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus Gustav Wieds Vej, 10C DK-8000, Aarhus C, Denmark aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 769-779 0167-4412 61:4-5<769 2006 61 11103 https://doi.org/10.1007/s11103-006-0048-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0048-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heckmann Anne Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus Gustav Wieds Vej, 10C DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Hebelstrup Kim Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus Gustav Wieds Vej, 10C DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Larsen Knud Department of Crop Physiology and Soil Science, Danish Institute of Agricultural Science, 50, DK-8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Micaelo Nuno Institutode Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, EAN, ITQB II, Piso 6, Apartado 127, 2781-901, Oeiras, Portugal aut NATIONALLICENCE 700 1- Jensen Erik Department of Molecular biology, Laboratory of Gene Expression, University of Aarhus Gustav Wieds Vej, 10C DK-8000, Aarhus C, Denmark aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 769-779 0167-4412 61:4-5<769 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer