caa a22 4500 467894108 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0050-7 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0050-7 Visualizing bz1 missense suppression in Zea mays [Elektronische Daten] an assay for monocot tRNA expression and utilization [Xing Wu, Zhihong Chen, Anuradha Shende, Hugo Dooner, William Folk] Bombardment of a highly expressed dicot tRNAala(GAC) gene into Zea mays bz-E2 or bz-E5 coleoptiles causes suppression of an Ala458 →Val missense mutation, visualized by the development of anthocyanin pigment. Missense suppression is blocked by mutation of tRNAala(GAC) at a site that prevents aminoacylation by the dicot alanyl-tRNA synthetase, indicating that features identified for expression and utilization of dicot tRNAs also function in monocots. This assay of the expression and utilization of tRNAala(GAC) also can be used to study a variety of tRNAs and their genes, most of which can be relatively easily altered to be charged by alanyl tRNA synthetase. Springer Science+Business Media B.V., 2006 Zea mays nationallicence bronze-1 nationallicence tRNA nationallicence Missense suppression nationallicence Alanyl tRNA synthetase nationallicence Wu Xing Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut Chen Zhihong Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut Shende Anuradha Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut Dooner Hugo Waksman Institute, Rutgers University, 08855, Piscataway, NJ, USA aut Folk William Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 795-798 0167-4412 61:4-5<795 2006 61 11103 https://doi.org/10.1007/s11103-006-0050-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0050-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Xing Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Chen Zhihong Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Shende Anuradha Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Dooner Hugo Waksman Institute, Rutgers University, 08855, Piscataway, NJ, USA aut NATIONALLICENCE 700 1- Folk William Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, 65211, Columbia, MO, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 795-798 0167-4412 61:4-5<795 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer