caa a22 4500 467894116 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0047-2 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0047-2 The auxin-induced K+ channel gene Zmk1 in maize functions in coleoptile growth and is required for embryo development [Elektronische Daten] [Katrin Philippar, Kai Büchsenschütz, David Edwards, Julia Löffler, Hartwig Lüthen, Erhard Kranz, Keith Edwards, Rainer Hedrich] The transcript level and in turn protein density of the K+-uptake channel ZMK1 in maize (Zea mays) coleoptiles is controlled by the phytohormone auxin. ZMK1 is involved in auxin-regulated coleoptile elongation as well as gravi- and phototropism. To provide unequivocal evidence for the role of ZMK1 in these elementary processes we screened for maize plants containing a Mutator-tagged Zmk1 gene. In a site-selected approach, we were able to identify three independent alleles of Mutator-transposon insertions in Zmk1. zmk1-m1::Mu1 plants were characterised by a Mu1 transposon inside intron 1 of ZMK1. When we analysed the Zmk1-transcript abundance in growing coleoptiles of these homozygous mutants, however, we found the K+-channel allele overexpressed. In consequence, elevated levels of K+-channel transcripts resulted in a growth phenotype as expected from more efficient K+-uptake, representing a central factor for turgor formation. Following Zmk1 expression during maize embryogenesis, we found this K+-channel gene constitutively expressed throughout embryo development and upregulated in late stages. In line with a vital role in embryogenesis, the mutations of exon 2 and intron 2 of Zmk1-zmk1-m2::Mu8 and zmk1-m3::MuA2-caused a lethal, defective-kernel phenotype. Thus, these results demonstrate the central role of the auxin-regulated K+-channel gene Zmk1 in coleoptile growth and embryo development. Springer Science+Business Media B.V., 2006 Auxin nationallicence Coleoptile growth nationallicence Embryo development nationallicence K+ channel nationallicence Maize nationallicence Mutator transposons nationallicence ZMK1 : Zea mays K+ channel 1 nationallicence Mutator : Mu nationallicence IAA : indole-3-acetic acid nationallicence TEA : tetraethylammonium nationallicence Philippar Katrin Julius-von-Sachs-Institut, Lehrstuhl Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, D-97082, Wuerzburg, Germany aut Büchsenschütz Kai Julius-von-Sachs-Institut, Lehrstuhl Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, D-97082, Wuerzburg, Germany aut Edwards David Primary Industries Research Victoria, Department of Primary Industries, La Trobe University, VIC 3086, Bundoora, Australia aut Löffler Julia Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, D-22609, Hamburg, Germany aut Lüthen Hartwig Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, D-22609, Hamburg, Germany aut Kranz Erhard Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, D-22609, Hamburg, Germany aut Edwards Keith School of Biological Sciences, University of Bristol, B58 1UG, Bristol, UK aut Hedrich Rainer Julius-von-Sachs-Institut, Lehrstuhl Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, D-97082, Wuerzburg, Germany aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 757-768 0167-4412 61:4-5<757 2006 61 11103 https://doi.org/10.1007/s11103-006-0047-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0047-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Philippar Katrin Julius-von-Sachs-Institut, Lehrstuhl Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, D-97082, Wuerzburg, Germany aut NATIONALLICENCE 700 1- Büchsenschütz Kai Julius-von-Sachs-Institut, Lehrstuhl Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, D-97082, Wuerzburg, Germany aut NATIONALLICENCE 700 1- Edwards David Primary Industries Research Victoria, Department of Primary Industries, La Trobe University, VIC 3086, Bundoora, Australia aut NATIONALLICENCE 700 1- Löffler Julia Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Lüthen Hartwig Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Kranz Erhard Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, D-22609, Hamburg, Germany aut NATIONALLICENCE 700 1- Edwards Keith School of Biological Sciences, University of Bristol, B58 1UG, Bristol, UK aut NATIONALLICENCE 700 1- Hedrich Rainer Julius-von-Sachs-Institut, Lehrstuhl Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, D-97082, Wuerzburg, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 757-768 0167-4412 61:4-5<757 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer