caa a22 4500 467894132 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0036-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0036-5 Germin-like genes are expressed during somatic embryogenesis and early development of conifers [Elektronische Daten] [M. Mathieu, M. Lelu-Walter, A. Blervacq, H. David, S. Hawkins, G. Neutelings] Germins and germin-like proteins (GLPs) are members of a superfamily of proteins widely distributed in plants. Their localization within the extracellular matrix and in some cases their hydrogen peroxide-producing activity suggests that these proteins are involved in cell wall metabolism during stress responses and developmental processes. Several very highly conserved conifer GLPs have been identified in somatic embryo tissues. In order to gain more knowledge on their potential involvement in the development of this particular tissue, we have characterized a new GLP gene, LmGER1 in hybrid larch. Anti-GLP immunserum and in-gel activity analyses suggested the presence of superoxide dismutase activity in apoplastic proteins from larch somatic embryos. These results could indicate a possible role for LmGER1 in this physiological process. The expression of LmGER1 has been followed during the maturation of somatic embryos and in different organs of young plantlets by homologous transformation with a promoter-gus construct. This promoter was activated in the root cap of young embryos and, later on, in the cotyledons and in the vascular procambium and xylem. Furthermore, the importance of this gene in embryo development was evaluated by transforming embryonal masses with a gene construct encoding a hairpin RNA leading to gene silencing. The potential role of LmGER1 in cross-linking of cell wall components is discussed. Springer Science+Business Media B.V., 2006 Cell wall nationallicence Conifers nationallicence Cross-linking nationallicence Germin-like proteins nationallicence Somatic embryogenesis nationallicence Superoxide dismutase nationallicence Mathieu M. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut Lelu-Walter M. Unité Amélioration, Génétique et Physiologie Forestières, INRA-CRO BP 20 619 Ardon, F-45166, Olivet Cedex, France aut Blervacq A. Laboratoire de Physiologie de la Différenciation Végétale UPRES EA3567, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut David H. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut Hawkins S. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut Neutelings G. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 615-627 0167-4412 61:4-5<615 2006 61 11103 https://doi.org/10.1007/s11103-006-0036-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0036-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mathieu M. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Lelu-Walter M. Unité Amélioration, Génétique et Physiologie Forestières, INRA-CRO BP 20 619 Ardon, F-45166, Olivet Cedex, France aut NATIONALLICENCE 700 1- Blervacq A. Laboratoire de Physiologie de la Différenciation Végétale UPRES EA3567, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- David H. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Hawkins S. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Neutelings G. Laboratoire de Physiologie des Parois Végétales UPRES EA3568-USC INRA, Université des Sciences et Technologies de Lille, Bât SN2, 59655, Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 615-627 0167-4412 61:4-5<615 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer