caa a22 4500 467894140 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0031-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-0031-x A Psb27 homologue in Arabidopsis thaliana is required for efficient repair of photodamaged photosystem II [Elektronische Daten] [Hua Chen, Dongyuan Zhang, Jinkui Guo, Hao Wu, Meifang Jin, Qingtao Lu, Congming Lu, Lixin Zhang] Psb27 has been identified as a lumenal protein associated with photosystem II (PSII). To gain insight into the function of Psb27, we isolated a mutant Arabidopsis plant with a loss of psb27 function. The quantity of PSII complexes and electron transfer within PSII remained largely unaffected in the psb27 mutant. Our results also showed that under high-light-illumination, PSII activity and the content of the PSII reaction center protein D1 decreased more significantly in the psb27 mutant than in wild-type (WT) plant. Treatment of leaves with a chloroplast protein synthesis inhibitor resulted in similar light-induced PSII inactivation levels and D1 protein degradation rates in the WT and psb27 mutant plants. Recovery of PSII activity after photoinhibition was delayed in the psb27 mutant, suggesting that Psb27 is required for efficient recovery of the photodamaged PSII complex. Overall, these results demonstrated that Psb27 in Arabidopsis is not essential for oxygenic photosynthesis and PSII formation. Instead, our results provide evidence for the involvement of this lumenal protein in the recovery process of PSII. Springer Science+Business Media B.V., 2006 Arabidopsis thaliana nationallicence Assembly nationallicence D1 Protein nationallicence Photosystem II nationallicence Photoinhibition nationallicence psb27 nationallicence Chen Hua Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut Zhang Dongyuan Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut Guo Jinkui Photosynthesis Research center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Wu Hao Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut Jin Meifang Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut Lu Qingtao Photosynthesis Research center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Lu Congming Photosynthesis Research center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Zhang Lixin Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 567-575 0167-4412 61:4-5<567 2006 61 11103 https://doi.org/10.1007/s11103-006-0031-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0031-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Hua Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Zhang Dongyuan Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Guo Jinkui Photosynthesis Research center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Wu Hao Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Jin Meifang Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Lu Qingtao Photosynthesis Research center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Lu Congming Photosynthesis Research center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Zhang Lixin Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 567-575 0167-4412 61:4-5<567 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer