caa a22 4500 467894167 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0046-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0046-3 Regulation of the kinase activity of the MIK GCK-like MAP4K by alternative splicing [Elektronische Daten] [Enric Castells, Pere Puigdomènech, Josep Casacuberta] Alternative splicing of introns is essential to ensure the complexity of mammalian genome functions. In particular, the generation of a high number of different isoforms by alternative splicing is an important characteristic of genes coding for signalling proteins such as mitogen activated protein kinases (MAPKs). This is thought to allow these proteins to transduce multiple stimuli in a highly regulated manner. Plant genes are also subjected to alternative splicing. Nevertheless, clear examples of the functional consequences of this phenomenon are still scarce in plants. MIK is a maize gene coding for a GCK-like MAP4K that can be activated by interaction with maize atypical receptor kinase (MARK), an atypical receptor kinase. Here we show that MIK is subjected to alternative splicing. Expression of MIK leads to, at least, 4 different mature mRNAs that accumulate with particular expression profiles during maize development. Our results show that the polypeptides encoded by the different MIK mRNAs display different kinase activity and are differentially activated by interaction with the MARK receptor. Two MIK isoforms display constitutive kinase activity, one isoform is inactive but can be activated by MARK, and the fourth MIK isoform is inactive and cannot be activated by MARK. Our results constitute a clear example of the biochemical consequences of alternative splicing in plants. The selective conservation during evolution of the intron-exon structure of the region coding for the regulator domain of MIK, as well as the maintenance in maize, rice and Arabidopsis of the alternative splicing of some of these introns, are strong indications of its functional importance. Springer Science+Business Media B.V., 2006 Alternative splicing nationallicence Intron retention nationallicence MAPK nationallicence MIK nationallicence Receptor kinase nationallicence Castells Enric Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18, 08034, Barcelona, Spain aut Puigdomènech Pere Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18, 08034, Barcelona, Spain aut Casacuberta Josep Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18, 08034, Barcelona, Spain aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 747-756 0167-4412 61:4-5<747 2006 61 11103 https://doi.org/10.1007/s11103-006-0046-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0046-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Castells Enric Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18, 08034, Barcelona, Spain aut NATIONALLICENCE 700 1- Puigdomènech Pere Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18, 08034, Barcelona, Spain aut NATIONALLICENCE 700 1- Casacuberta Josep Departament de Genètica Molecular, Laboratori de Genètica Molecular Vegetal CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18, 08034, Barcelona, Spain aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 747-756 0167-4412 61:4-5<747 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer