caa a22 4500 467894183 CHVBK 20180406152757.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0038-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0038-3 Identification of a dehydration and ABA-responsive promoter regulon and isolation of corresponding DNA binding proteins for the group 4 LEA gene CpC2 from C. plantagineum [Elektronische Daten] [Andrea Ditzer, Dorothea Bartels] The resurrection plant Craterostigma plantagineum (Scrophulariaceae) is used as a model system to investigate the molecular and biochemical basis of desiccation tolerance. Genes which contribute to desiccation tolerance are expressed during dehydration of this plant. One of the dehydration-induced genes is CpC2, a group 4 LEA gene. The CpC2 promoter was analysed and a core promoter region (CPR) was identified which is critical for the responsiveness of the gene to dehydration and the plant hormone ABA. The CPR motif contains two ABA-response elements (ABRE) and a binding site for HDZIP transcription factors. A yeast one-hybrid screen was performed to isolate CPR binding proteins. This resulted in the isolation of a bZIP transcription factor (CpbZIP1) and three highly conserved CpHistone H3 proteins. Two of these CpHistone H3 proteins are constitutively expressed histone H3 variants which are suggested to be involved in gene regulation via histone modification. The CpbZIP1 belongs to the group S of bZIP genes which possess long 5′-UTRs with a putative regulatory function. A second very similar bZIP clone, CpbZIP2, was isolated which contains a conserved small upstream open reading frame (uORF) within the 5′-leader sequence. A possible regulatory role of the uORF is discussed. Springer Science+Business Media B.V., 2006 Abscisic acid-responsive element (ABRE) nationallicence bZIP nationallicence Craterostigma plantagineum nationallicence Desiccation tolerance nationallicence Histone H3 nationallicence LEA gene promoter nationallicence ABA : Abscisic acid nationallicence ABRE : ABA-responsive element nationallicence bZIP : Basic region leucine zipper nationallicence CPR : Core promoter region nationallicence DRE : Dehydration-responsive element nationallicence GFP : Green fluorescent protein nationallicence GST : Gluthathione-S-transferase nationallicence GUS : β-Glucuronidase nationallicence HDZIP : Homeodomain leucine zipper nationallicence LEA : Late embryogenesis abundant nationallicence ORF : Open reading frame nationallicence uORF : Upstream ORF nationallicence 5′UTR : 5′-Untranslated region nationallicence Ditzer Andrea Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, D-53115, Bonn, Germany aut Bartels Dorothea Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, D-53115, Bonn, Germany aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 643-663 0167-4412 61:4-5<643 2006 61 11103 https://doi.org/10.1007/s11103-006-0038-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0038-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ditzer Andrea Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, D-53115, Bonn, Germany aut NATIONALLICENCE 700 1- Bartels Dorothea Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, D-53115, Bonn, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 643-663 0167-4412 61:4-5<643 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer