caa a22 4500 467894205 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0042-7 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0042-7 Effects of postharvest storage and dormancy status on ABA content, metabolism, and expression of genes involved in ABA biosynthesis and metabolism in potato tuber tissues [Elektronische Daten] [Luis Destefano-Beltrán, Donna Knauber, Linda Huckle, Jeffrey Suttle] At harvest, and for an indeterminate period thereafter, potato tubers will not sprout and are physiologically dormant. Abscisic acid (ABA) has been shown to play a critical role in tuber dormancy control but the mechanisms controlling ABA content during dormancy as well as the sites of ABA synthesis and catabolism are unknown. As a first step in defining the sites of synthesis and cognate processes regulating ABA turnover during storage and dormancy progression, gene sequences encoding the ABA biosynthetic enzymes zeaxanthin epoxidase (ZEP) and 9-cis-epoxycarotenoid dioxygenase (NCED) and three catabolism-related genes were used to quantify changes in their relative mRNA abundances in three specific tuber tissues (meristems, their surrounding periderm and underlying cortex) by qRT-PCR. During storage, StZEP expression was relatively constant in meristems, exhibited a biphasic pattern in periderm with transient increases during early and mid-to-late-storage, and peaked during mid-storage in cortex. Expression of two members of the potato NCED gene family was found to correlate with changes in ABA content in meristems (StNCED2) and cortex (StNCED1). Conversely, expression patterns of three putative ABA-8′-hydroxylase (CYP707A) genes during storage varied in a tissue-specific manner with expression of two of these genes rising in meristems and periderm and declining in cortex during storage. These results suggest that ABA synthesis and metabolism occur in all tuber tissues examined and that tuber ABA content during dormancy is the result of a balance of synthesis and metabolism that increasingly favors catabolism as dormancy ends and may be controlled at the level of StNCED and StCYP707A gene activities Springer Science+Business Media B.V., 2006 ABA nationallicence Dormancy nationallicence Gene expression nationallicence Potato nationallicence Solanum tuberosum L nationallicence Tuber nationallicence qRT-PCR nationallicence ABA : Abscisic acid nationallicence NCED : 9-cis-epoxycarotenoid dioxygenase nationallicence ZEP : Zeaxanthin epoxidase nationallicence Destefano-Beltrán Luis United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut Knauber Donna United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut Huckle Linda United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut Suttle Jeffrey United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 687-697 0167-4412 61:4-5<687 2006 61 11103 https://doi.org/10.1007/s11103-006-0042-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0042-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Destefano-Beltrán Luis United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut NATIONALLICENCE 700 1- Knauber Donna United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut NATIONALLICENCE 700 1- Huckle Linda United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut NATIONALLICENCE 700 1- Suttle Jeffrey United States Department of Agriculture, Agricultural Research Service, Sugarbeet and Potato Research Unit, Northern Crop Science Laboratory, 1307 18th Street N, State University Station, PO Box 5677, 58105, Fargo, ND, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 687-697 0167-4412 61:4-5<687 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer