caa a22 4500 467894213 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0030-y doi (NATIONALLICENCE)springer-10.1007/s11103-006-0030-y Identification and characterization of PiORP1, a Petunia oxysterol-binding-protein related protein involved in receptor-kinase mediated signaling in pollen, and analysis of the ORP gene family in Arabidopsis [Elektronische Daten] [Andrea Skirpan, Peter Dowd, Paja Sijacic, Cynthia Jaworski, Simon Gilroy, Teh-hui Kao] Oxysterol-binding proteins (OSBPs) and oxysterol-binding-protein related proteins (ORPs) are encoded by most eukaryotic genomes examined to date; however, they have not yet been characterized in plants. Here we report the identification and characterization of PiORP1, an ORP of Petunia inflata that interacts with the cytoplasmic kinase domain of a receptor-like kinase, named PRK1, of P. inflata. PiORP1 is phosphorylated by PRK1 in vitro and therefore may be involved in PRK1 signaling during pollen development and growth. RNA gel blot analysis showed that PiORP1 and PRK1 had very similar expression patterns in developing pollen, mature pollen and pollen tubes. GFP fusion proteins of PiORP1 localized in the plasma membrane of pollen tubes at distinct foci and its PH domain alone was sufficient to mediate this localization. The sequence for the oxysterol-binding domain of PiORP1 was used to search the genome of Arabidopsis; 12 ORPs were identified and phylogenetic analysis revealed that they fell into two distinct clades, consistent with the ORPs of other eukaryotes. RT-PCR analysis showed that all 12 Arabidopsis ORPs were expressed; 10 were expressed in most of the tissues examined under normal growth conditions, but only three were expressed in pollen. Springer Science+Business Media B.V., 2006 Oxysterol-binding-protein related proteins nationallicence Pollen nationallicence Pollen tube nationallicence Receptor-like kinase nationallicence ORP : oxysterol-binding-protein related protein nationallicence OSBP : oxysterol-binding protein nationallicence RT-PCR : reverse transcriptase polymerase chain reaction nationallicence Skirpan Andrea Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, 16802, University Park, PA, USA aut Dowd Peter Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, 16802, University Park, PA, USA aut Sijacic Paja Integrative Biosciences Graduate Program, The Pennsylvania State University, 16802, University Park, PA, USA aut Jaworski Cynthia National Eye Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut Gilroy Simon Department of Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut Kao Teh-hui Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, 16802, University Park, PA, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 553-565 0167-4412 61:4-5<553 2006 61 11103 https://doi.org/10.1007/s11103-006-0030-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0030-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Skirpan Andrea Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Dowd Peter Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Sijacic Paja Integrative Biosciences Graduate Program, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Jaworski Cynthia National Eye Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Gilroy Simon Department of Biology, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Kao Teh-hui Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 553-565 0167-4412 61:4-5<553 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer