caa a22 4500 46789423X CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s11103-006-0039-2 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0039-2 Chromatin immunoprecipitation analysis of the tobacco PR-1a - and the truncated CaMV 35S promoter reveals differences in salicylic acid-dependent TGA factor binding and histone acetylation [Elektronische Daten] [Thomas Butterbrodt, Corinna Thurow, Christiane Gatz] Salicylic acid (SA) is a plant signalling molecule needed for the induction of defence responses upon attack by a variety of pathogens. Truncation of the Cauliflower Mosaic Virus (CaMV) 35S promoter down to 90bp has identified activation sequence-1 (as-1) as an autonomous SA-responsive cis element. The as-1-like elements are found in a number of SA-inducible promoters like e.g. the tobacco PR-1a promoter. They are recognized by basic/leucine zipper (bZIP) transcription factors of the TGA family. In tobacco leaves, TGA2.2 is the most abundant TGA factor. TGA2.2 is required for the expression of as-1-containing promoters. Here we unravel clear differences between the "truncated” CaMV 35S and the PR-1a promoter with respect to in vivo TGA binding and histone acetylation. Chromatin immunoprecipitation (ChIP) analysis revealed SA-inducible recruitment of tobacco TGA2.2 as well as SA-inducible histone acetylation at the PR-1a promoter. In contrast, no influence of SA on TGA2.2 binding and histone acetylation was detectable at the "truncated” CaMV 35S promoter. The finding of SA-independent TGA factor binding in the absence of additional flanking regulatory sequences suggests that transcriptional activation is not necessarily mediated by inducible DNA binding of TGA factors. Plants with severely reduced TGA2.2 protein levels also showed SA-induced histone acetylation at the PR-1a promoter indicating that regulatory events independent from TGA2.2 function are initiated at the PR-1a promoter. Springer Science+Business Media B.V., 2006 Activating sequence-1 nationallicence Chromatin immunoprecipitation nationallicence Hyperacetylated histones nationallicence Pathogenesis related gene-1a nationallicence Salicylic acid nationallicence TGA factors nationallicence as-1 : activating sequence-1 nationallicence ASF-1 : as-1 binding factor-1 nationallicence α H4acetyl : antiserum against histone H4 acetylated at lysines 5, 8, 12 and 16 nationallicence α TGA2-C : antiserum against the bZIP domain and the C-terminal domain of TGA2.2 and TGA2.1 nationallicence α VP : antiserum against the activation domain of viral protein 16 of Herpes simplex virus nationallicence BSA : bovine serum albumin nationallicence bZIP : basic/leucine zipper nationallicence CaMV : Cauliflower Mosaic Virus nationallicence ChIP : chromatin immunoprecipitation nationallicence EMSA : electrophoretic mobility shift assay nationallicence GST : glutathione S-transferase nationallicence GUS : ß-glucoronidase nationallicence NPR1 : non-expressor of PR-genes nationallicence PAGE : polyacrylamide gel electrophoresis nationallicence PR : pathogenesis related nationallicence SA : salicylic acid nationallicence SAI : SA-inhibitor nationallicence SARP : salicylic acid responsive protein nationallicence SAR : systemic acquired resistance nationallicence SDS : sodium dodecyl sulphate nationallicence tc : tetracycline nationallicence tTA : tetracycline dependent trans-activator nationallicence UTR : untranslated region nationallicence 2.2VP : fusion protein of TGA2.2 with the activation domain of viral protein 16 of Herpes simplex virus nationallicence Butterbrodt Thomas Albrecht-von-Haller-Institut fuer Pflanzenwissenschaften, Georg-August-Universitaet Goettingen, Untere Karspuele 2, 37073, Goettingen, Germany aut Thurow Corinna Albrecht-von-Haller-Institut fuer Pflanzenwissenschaften, Georg-August-Universitaet Goettingen, Untere Karspuele 2, 37073, Goettingen, Germany aut Gatz Christiane Albrecht-von-Haller-Institut fuer Pflanzenwissenschaften, Georg-August-Universitaet Goettingen, Untere Karspuele 2, 37073, Goettingen, Germany aut Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 665-674 0167-4412 61:4-5<665 2006 61 11103 https://doi.org/10.1007/s11103-006-0039-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0039-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Butterbrodt Thomas Albrecht-von-Haller-Institut fuer Pflanzenwissenschaften, Georg-August-Universitaet Goettingen, Untere Karspuele 2, 37073, Goettingen, Germany aut NATIONALLICENCE 700 1- Thurow Corinna Albrecht-von-Haller-Institut fuer Pflanzenwissenschaften, Georg-August-Universitaet Goettingen, Untere Karspuele 2, 37073, Goettingen, Germany aut NATIONALLICENCE 700 1- Gatz Christiane Albrecht-von-Haller-Institut fuer Pflanzenwissenschaften, Georg-August-Universitaet Goettingen, Untere Karspuele 2, 37073, Goettingen, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/4-5(2006-07-01), 665-674 0167-4412 61:4-5<665 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer