caa a22 4500 467894248 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s11103-006-0054-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0054-3 Molecular analysis of early rice stamen developmentusing organ-specific gene expression profiling [Elektronische Daten] [Xiao-Chun Lu, Hua-Qin Gong, Mo-Li Huang, Su-Lan Bai, Yang-Bo He, Xizeng Mao, Zhi Geng, Song-Gang Li, Liping Wei, Jie-Shuai Yuwen, Zhi-Hong Xu, Shu-Nong Bai] Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series of events, with a given shape, structure and function. Research over the past two decades has elucidated the molecular mechanisms of organ identity and dorsalventral axis determinations. However, little is known about the molecular mechanisms underlying the successive processes. To develop an effective approach for studying organ formation at the molecular level, we generated organ-specific gene expression profiles (GEPs) reflecting early development in rice stamen. In this study, we demonstrated that the GEPs are highly correlated with early stamen development, suggesting that this analysis is useful for dissecting stamen development regulation. Based on the molecular and morphological correlation, we found that over 26 genes, that were preferentially up-regulated during early stamen development, may participate in stamen development regulation. In addition, we found that differentially expressed genes during early stamen development are clustered into two clades, suggesting that stamen development may comprise of two distinct phases of pattern formation and cellular differentiation. Moreover, the organ-specific quantitative changes in gene expression levels may play a critical role for regulating plant organ formation. Springer Science+Business Media B.V., 2006 Rice nationallicence Organ formation nationallicence Early stamen development nationallicence Organ-specific gene expression profiling nationallicence Microarray analysis nationallicence aRNA : antisense RNA nationallicence cDNA : complementary DNA nationallicence DEPC : diethyl pyrocarbonate nationallicence dUTP : deoxyuridine triphosphate nationallicence EMS : EXCESS MICROSPOROCYTES nationallicence ESTs : expression sequence tags nationallicence EXS : EXTRA SPOROGENOUS CELLS nationallicence FIL : FILAMENTOUS FLOWER nationallicence LLS : LETHAL LEAF SPOT nationallicence LPA : linear polyacrylamide nationallicence msca1 : male sterile converted anther1 nationallicence NZZ : NOZZLE nationallicence OsMADS7 : rice MADS-box protein 7 nationallicence PCR : polymerase chain reaction nationallicence PHD : plant homeodomain nationallicence RNA : ribosome nucleic acid nationallicence RT : reverse transcription nationallicence SPL : SPOROCYTELESS nationallicence TPD : TAPETUM DETERMINANT nationallicence Lu Xiao-Chun PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Gong Hua-Qin PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Huang Mo-Li PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Bai Su-Lan PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut He Yang-Bo Department of Probability and Statistics, School of Mathematical Sciences, Peking University, 100871, Beijing, China aut Mao Xizeng PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Geng Zhi Department of Probability and Statistics, School of Mathematical Sciences, Peking University, 100871, Beijing, China aut Li Song-Gang PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Wei Liping PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Yuwen Jie-Shuai PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Xu Zhi-Hong PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Bai Shu-Nong PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 845-861 0167-4412 61:6<845 2006 61 11103 https://doi.org/10.1007/s11103-006-0054-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0054-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Xiao-Chun PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Gong Hua-Qin PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Huang Mo-Li PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Bai Su-Lan PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- He Yang-Bo Department of Probability and Statistics, School of Mathematical Sciences, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Mao Xizeng PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Geng Zhi Department of Probability and Statistics, School of Mathematical Sciences, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Li Song-Gang PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Wei Liping PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Yuwen Jie-Shuai PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Xu Zhi-Hong PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 700 1- Bai Shu-Nong PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, 100871, Beijing, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 845-861 0167-4412 61:6<845 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer