caa a22 4500 467894299 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s11103-006-0055-2 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0055-2 Differential gene expression of wheat progeny with contrasting levels of transpiration efficiency [Elektronische Daten] [Gang-Ping Xue, C. McIntyre, Scott Chapman, Neil Bower, Heather Way, Antonio Reverter, Bryan Clarke, Ray Shorter] High water use efficiency or transpiration efficiency (TE) in wheat is a desirable physiological trait for increasing grain yield under water-limited environments. The identification of genes associated with this trait would facilitate the selection for genotypes with higher TE using molecular markers. We performed an expression profiling (microarray) analysis of approximately 16,000 unique wheat ESTs to identify genes that were differentially expressed between wheat progeny lines with contrasting TE levels from a cross between Quarrion (high TE) and Genaro 81 (low TE). We also conducted a second microarray analysis to identify genes responsive to drought stress in wheat leaves. Ninety-three genes that were differentially expressed between high and low TE progeny lines were identified. One fifth of these genes were markedly responsive to drought stress. Several potential growth-related regulatory genes, which were down-regulated by drought, were expressed at a higher level in the high TE lines than the low TE lines and are potentially associated with a biomass production component of the Quarrion-derived high TE trait. Eighteen of the TE differentially expressed genes were further analysed using quantitative RT-PCR on a separate set of plant samples from those used for microarray analysis. The expression levels of 11 of the 18 genes were positively correlated with the high TE trait, measured as carbon isotope discrimination (Δ13C). These data indicate that some of these TE differentially expressed genes are candidates for investigating processes that underlie the high TE trait or for use as expression quantitative trait loci (eQTLs) for TE. Springer Science+Business Media B.V., 2006 Biomass production nationallicence Drought nationallicence Gene expression nationallicence Microarray nationallicence Transpiration efficiency nationallicence Wheat nationallicence ABA : abscisic acid nationallicence eQTL : expression quantitative trait locus nationallicence EST : expressed sequence tag nationallicence QTL : quantitative trait locus nationallicence RLWC : relative leaf water content nationallicence RT-PCR : reverse transcription-polymerase chain reaction nationallicence TE : transpiration efficiency nationallicence TPR : tetratricopeptide repeat nationallicence Xue Gang-Ping CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut McIntyre C. CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut Chapman Scott CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut Bower Neil CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut Way Heather CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut Reverter Antonio CSIRO Livestock Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut Clarke Bryan CSIRO Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia aut Shorter Ray CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 863-881 0167-4412 61:6<863 2006 61 11103 https://doi.org/10.1007/s11103-006-0055-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0055-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xue Gang-Ping CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 700 1- McIntyre C. CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 700 1- Chapman Scott CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 700 1- Bower Neil CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 700 1- Way Heather CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 700 1- Reverter Antonio CSIRO Livestock Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 700 1- Clarke Bryan CSIRO Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia aut NATIONALLICENCE 700 1- Shorter Ray CSIRO Plant Industry, 306 Carmody Road, 4067, St Lucia, Qld, Australia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 863-881 0167-4412 61:6<863 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer