caa a22 4500 467894310 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s11103-006-0058-z doi (NATIONALLICENCE)springer-10.1007/s11103-006-0058-z Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides [Elektronische Daten] [Gang Pan, Xianyin Zhang, Kede Liu, Jiwen Zhang, Xiaozhi Wu, Jun Zhu, Jumin Tu] Development of hybrid rice has greatly contributed to increased yields during the past three decades. Two bentazon-lethal mutants 8077S and Norin8m are being utilized in developing new hybrid rice systems. When the male sterile lines are developed in such a mutant background, the problem of F1 seed contamination by self-seeds from the sterile lines can be solved by spraying bentazon at seedling stage. We first determined the sensitivity of the mutant plants to bentazon. Both mutants showed symptoms to bentazon starting from 100mg/l, which was about 60-fold, lower than the sensitivity threshold of their wild-type controls. In addition, both mutants were sensitive to sulfonylurea-type herbicides. The locus for the mutant phenotype is bel for 8077S and bsl for Norin8m. Tests showed that the two loci are allelic to each other. The two genes were cloned by map-based cloning. Interestingly, both mutant alleles had a single-base deletion, which was confirmed by PCR-RFLP. The two loci are renamed bel a (for bel) and bel b (for bsl). The wild-type Bel gene encodes a novel cytochrome P450 monooxgenase, named CYP81A6. Analysis of the mutant protein sequence also revealed the reason for bel a being slightly tolerant than bel b . Introduction of the wild-type Bel gene rescued the bentazon- and sulfonylurea-sensitive phenotype of bel a mutant. On the other hand, expression of antisense Bel in W6154S induced a mutant phenotype. Based on these results we conclude that the novel cytochrome P450 monooxygenase CYP81A6 encoded by Bel confers resistance to two different classes of herbicides. Springer Science+Business Media B.V., 2006 bel a nationallicence bel b nationallicence CYP81A6 nationallicence Herbicides resistance nationallicence Map-based cloning nationallicence Oryza sativa L nationallicence Pan Gang College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut Zhang Xianyin College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut Liu Kede National Key Laboratory of Crop Genetic Improvement, 430070, Wuhan, Hubei, China aut Zhang Jiwen Institue of Crop Breeding and Cultivation, Hubei Academy of Agricultural Science, 430064, Wuhan, Hubei, China aut Wu Xiaozhi Institue of Crop Breeding and Cultivation, Hubei Academy of Agricultural Science, 430064, Wuhan, Hubei, China aut Zhu Jun College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut Tu Jumin College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 933-943 0167-4412 61:6<933 2006 61 11103 https://doi.org/10.1007/s11103-006-0058-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0058-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pan Gang College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Zhang Xianyin College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Liu Kede National Key Laboratory of Crop Genetic Improvement, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhang Jiwen Institue of Crop Breeding and Cultivation, Hubei Academy of Agricultural Science, 430064, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wu Xiaozhi Institue of Crop Breeding and Cultivation, Hubei Academy of Agricultural Science, 430064, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhu Jun College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Tu Jumin College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 933-943 0167-4412 61:6<933 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer