caa a22 4500 467894329 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s11103-006-0056-1 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0056-1 Synergy between the light-induced acute response and the circadian cycle: a new mechanism for the synchronization of the Phaseolus vulgaris clock to light [Elektronische Daten] [Athanasios-Dimitrios Kaldis, Anastasia Prombona] PvLHY and Lhcb expression has been studied in primary bean leaves after exposure of etiolated leaves to two or three white light-pulses and under different photoperiods. Under the tested photoperiods, the steady-state mRNA levels exhibit diurnal oscillations with zenith in the morning between ZT21 and 4 for PvLHY and between ZT4 and 6 for Lhcb. Nadir is in the evening between ZT12 and 18 for PvLHY and ZT18 and 24 for Lhcb. Light-pulses to etiolated seedlings induce a differentiated acute response that is reciprocally correlated with the amplitude of the following circadian cycle. In addition, the clock modulates the duration of the acute response (descending part of the curve included), which according to the phase of the rhythm at light application extends from 7 to 18h. This constitutes the response dynamics of the Phaseolus clock to light. Similarly, the waveform of PvLHY and Lhcb expression during the day of different photoperiods resembles in induction capability (accomplishment of peak after lights-on) and duration (from lights-on phase to trough) the phase-dependent progression of acute response in etiolated seedlings. Consequently, the peak of Lhcb (all tested photoperiods) and PvLHY (in LD 18:6) attained in the photophase corresponds to the acute response peak, while the peak of PvLHY during the scotophase (in LD 12:12 and 6:18) corresponds to the circadian peak. Thus, the effect of the response dynamics in the photoperiod determines the coincidence of the peak with the photo- or scotophase, respectively. This represents a new model mechanism for the adaptation of the Phaseolus clock to light. Springer Science+Business Media B.V., 2006 Acute response nationallicence Circadian cycle nationallicence Lhcb nationallicence Clock synchronization nationallicence PvLHY nationallicence Lhcb : Genes encoding the apoproteins of PSII light harvesting complexes (LHCB) nationallicence PvLHY : The ortholog clock gene of Arabidopsis thaliana LHY (LATE ELONGATED HYPOCOTYL) in Phaseolus vulgaris nationallicence Kaldis Athanasios-Dimitrios Institute of Biology, NCSR ‘Demokritos', 15310 Aghia Paraskevi, Attiki, Greece aut Prombona Anastasia Institute of Biology, NCSR ‘Demokritos', 15310 Aghia Paraskevi, Attiki, Greece aut Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 883-895 0167-4412 61:6<883 2006 61 11103 https://doi.org/10.1007/s11103-006-0056-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0056-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaldis Athanasios-Dimitrios Institute of Biology, NCSR ‘Demokritos', 15310 Aghia Paraskevi, Attiki, Greece aut NATIONALLICENCE 700 1- Prombona Anastasia Institute of Biology, NCSR ‘Demokritos', 15310 Aghia Paraskevi, Attiki, Greece aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 883-895 0167-4412 61:6<883 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer