caa a22 4500 467894353 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s11103-006-0052-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0052-5 Identification and functional characterization of a leucine-rich repeat receptor-like kinase gene that is involved in regulation of soybean leaf senescence [Elektronische Daten] [Xiao-Ping Li, Rui Gan, Peng-Li Li, Yuan-Yuan Ma, Li-Wen Zhang, Ren Zhang, Yong Wang, Ning Wang] We report here the cloning and characterization of a soybean receptor-like kinase (RLK) gene, designated GmSARK (Glycine max senescence-associated receptor-like kinase), which is involved in regulating leaf senescence. The conceptual protein product of GmSARK contains typical domains of LRR receptor-like kinases: a cytoplasmic domain with all the 11 kinase subdomains, a transmembrane domain and an extracelullar domain containing 9 Leucine-Rich Repeat (LRR) units that may act as a receptor. The expression of GmSARK in soybean leaves was up-regulated in all the three tested senescence systems: senescing cotyledons, dark-induced primary leaf senescence and the natural leaf senescence process after florescence. Furthermore, the RNA interference (RNAi)-mediated knocking-down of GmSARK dramatically retarded soybean leaf senescence. A more complex thylakoid membrane system, higher foliar level of chlorophyll content and a very remarkable delay of senescence-induced disintegration of chloroplast structure were observed in GmSARK-RNAi transgenic leaves. A homolog of maize lethal leaf-spot 1 gene, which has been suggested to encode a key enzyme catalyzing chlorophyll breakdown, was isolated and nominated Gmlls1. The expression level of Gmgtr1 gene, which encodes a key enzyme of chlorophyll synthesis, was also analyzed. It was found that Gmlls1 was up-regulated and Gmgtr1 was down-regulated during senescence in wild-type soybean leaves. However, both of the up-regulation of Gmlls1 and down-regulation of Gmgtr1 were retarded during senescence of GmSARK-RNAi transgenic leaves. In addition, over-expression of the GmSARK gene greatly accelerated the senescence progression of CaMV 35S:GmSARK transgenic plants. Taken together, these results strongly suggested the involvement of this LRR-RLK in regulation of soybean leaf senescence, maybe via regulating chloroplast development and chlorophyll accumulation. Multiple functions of GmSARK besides its regulation of leaf senescence were also discussed. Springer Science+Business Media B.V., 2006 Knock-down nationallicence Leaf senescence nationallicence Over-expression nationallicence Receptor-like kinase nationallicence RNA interference nationallicence Soybean nationallicence RLK : Receptor-like kinase nationallicence SAG : Senescence-associated gene nationallicence RNAi : RNA interference nationallicence LRR : Leucine-rich repeats nationallicence GmSARK : Glycine max senescence-associated receptor-like kinase nationallicence Gmgtr1 : Glycine max glutamyl-tRNA reductase nationallicence Gmlls1 : Glycine max lethal leaf-spot 1 nationallicence Li Xiao-Ping Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Gan Rui Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Li Peng-Li Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Ma Yuan-Yuan Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Zhang Li-Wen Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Zhang Ren School of Biological Sciences, University of Wollongong, NSW 2522, Wollongong, Australia aut Wang Yong Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Wang Ning Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 829-844 0167-4412 61:6<829 2006 61 11103 https://doi.org/10.1007/s11103-006-0052-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0052-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiao-Ping Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 700 1- Gan Rui Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 700 1- Li Peng-Li Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 700 1- Ma Yuan-Yuan Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 700 1- Zhang Li-Wen Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 700 1- Zhang Ren School of Biological Sciences, University of Wollongong, NSW 2522, Wollongong, Australia aut NATIONALLICENCE 700 1- Wang Yong Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 700 1- Wang Ning Department of Plant Biology and Ecology, Nankai University, 300071, Tianjin, P.R. China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/6(2006-08-01), 829-844 0167-4412 61:6<829 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer