caa a22 4500 467894361 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0027-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0027-6 High Lysine and High Tryptophan Transgenic Maize Resulting from the Reduction of Both 19-and 22-kD α-zeins [Elektronische Daten] [Shihshieh Huang, Alessandra Frizzi, Cheryl Florida, Diane Kruger, Michael Luethy] The major maize seed storage proteins, zeins, are deficient in lysine and tryptophan content, which contribute to the poor nutritional quality of corn. Whether through the identification of mutations or genetic engineering, kernels with reduced levels of zein proteins have been shown to have increased levels of lysine and tryptophan. It has been hypothesized that these increases are due to the reduction of lysine-poor zeins and a pleiotropic increase in the lysine-rich non-zein proteins. By transforming maize with constructs expressing chimeric double-stranded RNA, kernels derived from stable transgenic plants displayed significant declines in the accumulation of both 19-and 22-kD α-zeins, which resulted in higher lysine and tryptophan content than previously reported for kernels with reduced zein levels. The observation that lysine and tryptophan content is correlated with the protein levels measured in transgenic maize kernels is consistent with the hypothesis that a pleiotropic increase in non-zein proteins is contributing to an improved amino acid balance. In addition, a large increase in accumulation of free amino acids, consisting predominantly of asparagine, asparate and glutamate, was observed in the zein reduction kernels. Springer, 2006 gene suppression nationallicence high lysine nationallicence high tryptophan nationallicence maize nutritional quality nationallicence maize quality trait nationallicence zein reduction nationallicence Huang Shihshieh Mystic Research, Monsanto Company, 62 Maritime Drive, 06355, Mystic, CT, USA aut Frizzi Alessandra Mystic Research, Monsanto Company, 62 Maritime Drive, 06355, Mystic, CT, USA aut Florida Cheryl Chesterfield Village Campus, Monsanto Company, 700 Chesterfield Parkway West, 63017, Chesterfield, MO, USA aut Kruger Diane Mystic Research, Monsanto Company, 62 Maritime Drive, 06355, Mystic, CT, USA aut Luethy Michael Chesterfield Village Campus, Monsanto Company, 700 Chesterfield Parkway West, 63017, Chesterfield, MO, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 525-535 0167-4412 61:3<525 2006 61 11103 https://doi.org/10.1007/s11103-006-0027-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0027-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Shihshieh Mystic Research, Monsanto Company, 62 Maritime Drive, 06355, Mystic, CT, USA aut NATIONALLICENCE 700 1- Frizzi Alessandra Mystic Research, Monsanto Company, 62 Maritime Drive, 06355, Mystic, CT, USA aut NATIONALLICENCE 700 1- Florida Cheryl Chesterfield Village Campus, Monsanto Company, 700 Chesterfield Parkway West, 63017, Chesterfield, MO, USA aut NATIONALLICENCE 700 1- Kruger Diane Mystic Research, Monsanto Company, 62 Maritime Drive, 06355, Mystic, CT, USA aut NATIONALLICENCE 700 1- Luethy Michael Chesterfield Village Campus, Monsanto Company, 700 Chesterfield Parkway West, 63017, Chesterfield, MO, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 525-535 0167-4412 61:3<525 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer